Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers

Amanda T. Dang, Wei He, Daniela B. Ivey, Matthew A. Coleman, Tonya L. Kuhl

Research output: Contribution to journalArticle

Abstract

A nanolipoprotein particle (NLP) is a lipid bilayer disc stabilized by two amphipathic "scaffold" apolipoproteins. It has been most notably utilized as a tool for solubilizing a variety of membrane proteins while preserving structural and functional properties. Transfer of functional proteins from NLPs into model membrane systems such as supported lipid bilayers (SLBs) would enable new opportunities, for example, two-dimensional protein crystallization and studies on protein-protein interactions. This work used fluorescence microscopy and atomic force microscopy to investigate the interaction between NLPs and SLBs. When incubated with SLBs, NLPs were found to spontaneously deliver lipid and protein cargo. The impact of membrane composition on lipid exchange was explored, revealing a positive correlation between the magnitude of lipid transfer and concentration of defects in the target SLB. Incorporation of lipids capable of binding specifically to polyhistidine tags encoded into the apolipoproteins also boosted transfer of NLP cargo. Optimal conditions for lipid and protein delivery from NLPs to SLBs are proposed based on interaction mechanisms.

Original languageEnglish (US)
Pages (from-to)12071-12078
Number of pages8
JournalLangmuir
Volume35
Issue number37
DOIs
StatePublished - Sep 17 2019
Externally publishedYes

Fingerprint

Lipid bilayers
Lipids
lipids
proteins
Proteins
Apolipoproteins
Membranes
cargo
Fluorescence microscopy
membranes
Crystallization
Scaffolds
lipid transfer protein
Atomic force microscopy
Membrane Proteins
Defects
interactions
preserving
Chemical analysis
delivery

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers. / Dang, Amanda T.; He, Wei; Ivey, Daniela B.; Coleman, Matthew A.; Kuhl, Tonya L.

In: Langmuir, Vol. 35, No. 37, 17.09.2019, p. 12071-12078.

Research output: Contribution to journalArticle

Dang, Amanda T. ; He, Wei ; Ivey, Daniela B. ; Coleman, Matthew A. ; Kuhl, Tonya L. / Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers. In: Langmuir. 2019 ; Vol. 35, No. 37. pp. 12071-12078.
@article{a7f22d7932c24c3dba543c979b9f44dc,
title = "Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers",
abstract = "A nanolipoprotein particle (NLP) is a lipid bilayer disc stabilized by two amphipathic {"}scaffold{"} apolipoproteins. It has been most notably utilized as a tool for solubilizing a variety of membrane proteins while preserving structural and functional properties. Transfer of functional proteins from NLPs into model membrane systems such as supported lipid bilayers (SLBs) would enable new opportunities, for example, two-dimensional protein crystallization and studies on protein-protein interactions. This work used fluorescence microscopy and atomic force microscopy to investigate the interaction between NLPs and SLBs. When incubated with SLBs, NLPs were found to spontaneously deliver lipid and protein cargo. The impact of membrane composition on lipid exchange was explored, revealing a positive correlation between the magnitude of lipid transfer and concentration of defects in the target SLB. Incorporation of lipids capable of binding specifically to polyhistidine tags encoded into the apolipoproteins also boosted transfer of NLP cargo. Optimal conditions for lipid and protein delivery from NLPs to SLBs are proposed based on interaction mechanisms.",
author = "Dang, {Amanda T.} and Wei He and Ivey, {Daniela B.} and Coleman, {Matthew A.} and Kuhl, {Tonya L.}",
year = "2019",
month = "9",
day = "17",
doi = "10.1021/acs.langmuir.9b01288",
language = "English (US)",
volume = "35",
pages = "12071--12078",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "37",

}

TY - JOUR

T1 - Lipid and Protein Transfer between Nanolipoprotein Particles and Supported Lipid Bilayers

AU - Dang, Amanda T.

AU - He, Wei

AU - Ivey, Daniela B.

AU - Coleman, Matthew A.

AU - Kuhl, Tonya L.

PY - 2019/9/17

Y1 - 2019/9/17

N2 - A nanolipoprotein particle (NLP) is a lipid bilayer disc stabilized by two amphipathic "scaffold" apolipoproteins. It has been most notably utilized as a tool for solubilizing a variety of membrane proteins while preserving structural and functional properties. Transfer of functional proteins from NLPs into model membrane systems such as supported lipid bilayers (SLBs) would enable new opportunities, for example, two-dimensional protein crystallization and studies on protein-protein interactions. This work used fluorescence microscopy and atomic force microscopy to investigate the interaction between NLPs and SLBs. When incubated with SLBs, NLPs were found to spontaneously deliver lipid and protein cargo. The impact of membrane composition on lipid exchange was explored, revealing a positive correlation between the magnitude of lipid transfer and concentration of defects in the target SLB. Incorporation of lipids capable of binding specifically to polyhistidine tags encoded into the apolipoproteins also boosted transfer of NLP cargo. Optimal conditions for lipid and protein delivery from NLPs to SLBs are proposed based on interaction mechanisms.

AB - A nanolipoprotein particle (NLP) is a lipid bilayer disc stabilized by two amphipathic "scaffold" apolipoproteins. It has been most notably utilized as a tool for solubilizing a variety of membrane proteins while preserving structural and functional properties. Transfer of functional proteins from NLPs into model membrane systems such as supported lipid bilayers (SLBs) would enable new opportunities, for example, two-dimensional protein crystallization and studies on protein-protein interactions. This work used fluorescence microscopy and atomic force microscopy to investigate the interaction between NLPs and SLBs. When incubated with SLBs, NLPs were found to spontaneously deliver lipid and protein cargo. The impact of membrane composition on lipid exchange was explored, revealing a positive correlation between the magnitude of lipid transfer and concentration of defects in the target SLB. Incorporation of lipids capable of binding specifically to polyhistidine tags encoded into the apolipoproteins also boosted transfer of NLP cargo. Optimal conditions for lipid and protein delivery from NLPs to SLBs are proposed based on interaction mechanisms.

UR - http://www.scopus.com/inward/record.url?scp=85072288760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072288760&partnerID=8YFLogxK

U2 - 10.1021/acs.langmuir.9b01288

DO - 10.1021/acs.langmuir.9b01288

M3 - Article

C2 - 31442053

AN - SCOPUS:85072288760

VL - 35

SP - 12071

EP - 12078

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 37

ER -