Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome

Ram Reifen, Howard Amital, Miri Blank, David Sklan, Zipi Berkovich, M. Eric Gershwin, Yehuda Shoenfeld

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naive mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-βGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-βGPI were significantly lower (in O.D. at 405 nm, 1.387±0.232 in comparison to non-treated mice 0.637±0.111, P<0.05). The reduced titer of anti-βGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-βGPI forming cells in cultured splenocytes (84±14, antibody-forming cells (AFC)/105 cells in comparison to 37±4 AFC/105 cells (P<0.02). In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-βGPI was lower in comparison to mice immunized with H-3 anti-βGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalJournal of Autoimmunity
Volume15
Issue number3
DOIs
StatePublished - 2000

Fingerprint

beta 2-Glycoprotein I
Linseed Oil
Antiphospholipid Syndrome
Diet
Antibodies
Omega-3 Fatty Acids
Unsaturated Fatty Acids
Cultured Cells
Vaccination
Monoclonal Antibodies
T-Lymphocytes
Control Groups
Serum

Keywords

  • Anti-beta-2-glycoprotein-I antibodies
  • Antiphospholipid syndrome
  • Autoantibodies
  • Autoimmune diseases
  • Nutrition
  • Polyunsaturated fatty acids

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome. / Reifen, Ram; Amital, Howard; Blank, Miri; Sklan, David; Berkovich, Zipi; Gershwin, M. Eric; Shoenfeld, Yehuda.

In: Journal of Autoimmunity, Vol. 15, No. 3, 2000, p. 381-385.

Research output: Contribution to journalArticle

Reifen, Ram ; Amital, Howard ; Blank, Miri ; Sklan, David ; Berkovich, Zipi ; Gershwin, M. Eric ; Shoenfeld, Yehuda. / Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome. In: Journal of Autoimmunity. 2000 ; Vol. 15, No. 3. pp. 381-385.
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