Abstract
The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naive mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-βGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-βGPI were significantly lower (in O.D. at 405 nm, 1.387±0.232 in comparison to non-treated mice 0.637±0.111, P<0.05). The reduced titer of anti-βGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-βGPI forming cells in cultured splenocytes (84±14, antibody-forming cells (AFC)/105 cells in comparison to 37±4 AFC/105 cells (P<0.02). In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-βGPI was lower in comparison to mice immunized with H-3 anti-βGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS. (C) 2000 Academic Press.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 381-385 |
| Number of pages | 5 |
| Journal | Journal of Autoimmunity |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2000 |
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Keywords
- Anti-beta-2-glycoprotein-I antibodies
- Antiphospholipid syndrome
- Autoantibodies
- Autoimmune diseases
- Nutrition
- Polyunsaturated fatty acids
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome. / Reifen, Ram; Amital, Howard; Blank, Miri; Sklan, David; Berkovich, Zipi; Gershwin, M. Eric; Shoenfeld, Yehuda.
In: Journal of Autoimmunity, Vol. 15, No. 3, 2000, p. 381-385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Linseed oil suppresses the anti-beta-2-glycoprotein-I in experimental antiphospholipid syndrome
AU - Reifen, Ram
AU - Amital, Howard
AU - Blank, Miri
AU - Sklan, David
AU - Berkovich, Zipi
AU - Gershwin, M. Eric
AU - Shoenfeld, Yehuda
PY - 2000
Y1 - 2000
N2 - The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naive mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-βGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-βGPI were significantly lower (in O.D. at 405 nm, 1.387±0.232 in comparison to non-treated mice 0.637±0.111, P<0.05). The reduced titer of anti-βGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-βGPI forming cells in cultured splenocytes (84±14, antibody-forming cells (AFC)/105 cells in comparison to 37±4 AFC/105 cells (P<0.02). In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-βGPI was lower in comparison to mice immunized with H-3 anti-βGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS. (C) 2000 Academic Press.
AB - The immunomodulatory potential of a diet enriched with n-3 polyunsaturated fatty acids was analysed in naive mice with experimental antiphospholipid syndrome (APS) induced by active immunization with H-3, a human anti-beta-2-glycoprotein-I (anti-βGPI) monoclonal antibody (mAb). Fetal loss and other clinical manifestations of APS were prevented in the group of APS mice upon exposure to the enriched n-3 diet compared to the control group. The titers of anti-βGPI were significantly lower (in O.D. at 405 nm, 1.387±0.232 in comparison to non-treated mice 0.637±0.111, P<0.05). The reduced titer of anti-βGPI antibodies in the sera of the treated mice was associated with a reduced number of anti-βGPI forming cells in cultured splenocytes (84±14, antibody-forming cells (AFC)/105 cells in comparison to 37±4 AFC/105 cells (P<0.02). In addition to the suppression of the humoral response in mice with experimental APS fed with linseed oil, we also observed an inhibitory effect on the cellular response. The T-cell response to anti-βGPI was lower in comparison to mice immunized with H-3 anti-βGPI mAB, which were kept on a normal diet. These results indicate that polyunsaturated fatty acids may improve clinical and laboratory parameters of APS. The beneficial effects of diets enriched with n-3 should be further examined as a potential mode of therapy for patients with APS. (C) 2000 Academic Press.
KW - Anti-beta-2-glycoprotein-I antibodies
KW - Antiphospholipid syndrome
KW - Autoantibodies
KW - Autoimmune diseases
KW - Nutrition
KW - Polyunsaturated fatty acids
UR - http://www.scopus.com/inward/record.url?scp=0033712602&partnerID=8YFLogxK
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U2 - 10.1006/jaut.2000.0439
DO - 10.1006/jaut.2000.0439
M3 - Article
VL - 15
SP - 381
EP - 385
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 3
ER -