Linoleic acid-derived 13-hydroxyoctadecadienoic acid is absorbed and incorporated into rat tissues

Zhichao Zhang, Shiva Emami, Marie Hennebelle, Rhianna K. Morgan, Larry A. Lerno, Carolyn M. Slupsky, Pamela J. Lein, Ameer Y. Taha

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Linoleic acid (LNA)-derived 13-hydroxyoctadecadienoic acid (13-HODE) is a bioactive lipid mediator that regulates multiple signaling processes in vivo. 13-HODE is also produced when LNA is oxidized during food processing. However, the absorption and incorporation kinetics of dietary 13-HODE into tissues is not known. The present study measured unesterified d4-13-HODE plasma bioavailability and incorporation into rat liver, adipose, heart and brain following gavage or intravenous (IV) injection (n = 3 per group). Mass spectrometry analysis revealed that d4-13-HODE was absorbed within 20 min of gavage, and continued to incorporate into plasma esterified lipid fractions throughout the 90 min monitoring period (incorporation half-life of 71 min). Following IV injection, unesterified d4-13-HODE was rapidly eliminated from plasma with a half-life of 1 min. Analysis of tracer incorporation kinetics into rat tissues following IV injection or gavage revealed that the esterified tracer preferentially incorporated into liver, adipose and heart compared to unesterified d4-13-HODE. No tracer was detected in the brain. This study demonstrates that dietary 13-HODE is absorbed, and incorporated into peripheral tissues from esterified plasma lipid pools. Understanding the chronic effects of dietary 13-HODE exposure on peripheral tissue physiology and metabolism merits future investigation.

Original languageEnglish (US)
Article number158870
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Issue number3
StatePublished - Mar 2021


  • Adipose tissue
  • Brain
  • Heart
  • Kinetics
  • Liver
  • Oxidized linoleic acid metabolites (OXLAMs)

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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