Linking molecular and population stress responses in Daphnia magna exposed to cadmium

Richard E Connon, Helen L. Hooper, Richard M. Sibly, Fei Ling Lim, Lars Henrik Heckmann, David J. Moore, Hajime Watanabe, Anneleen Soetaert, Katie Cook, Steve J. Maund, Thomas H. Hutchinson, Jonathan Moggs, Wim De Coen, Taisen Iguchi, Amanda Callaghan

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

DNA microarrays can be used to measure environmental stress responses. If they are to be predictive of environmental impact, we need to determine if altered gene expression translates into negative impacts on individuals and populations. A large cDNA microarray (14000 spots) was created to measure molecular stress responses to cadmium in Daphnia magnate most widely used aquatic indicator species, and relate responses to population growth rate (pgr). We used the array to detect differences in the transcription of genes in juvenile D. magna (24 h old) after 24 h exposure to a control and three cadmium concentrations (6, 20, and 37 μg Cd2+ L-1). Stress responses at the population level were estimated following a further 8 days exposure. Pgr was approximately linear negative with increasing cadmium concentration over this range. The microarray profile of gene expression in response to acute cadmium exposure begins to provide an overview of the molecular responses of D. magna, especially in relation to growth and development. Of the responding genes, 29% were involved with metabolism including carbohydrate, fat and peptide metabolism, and energy production, 31% were involved with transcription/translation, while 40% of responding genes were associated with cellular processes like growth and moulting, ion transport, and general stress responses (which included oxidative stress). Our production and application of a large Daphnia magna microarray has shown that measured gene responses can be logically linked to the impact of a toxicant such as cadmium on somatic growth and development, and consequently pgr.

Original languageEnglish (US)
Pages (from-to)2181-2188
Number of pages8
JournalEnvironmental Science and Technology
Volume42
Issue number6
DOIs
StatePublished - Mar 15 2008

Fingerprint

Cadmium
Microarrays
cadmium
Genes
gene
Transcription
Gene expression
growth and development
gene expression
population growth
metabolism
Oxidative stress
environmental stress
Oils and fats
Metabolism
peptide
Peptides
fat
Environmental impact
carbohydrate

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Cite this

Connon, R. E., Hooper, H. L., Sibly, R. M., Lim, F. L., Heckmann, L. H., Moore, D. J., ... Callaghan, A. (2008). Linking molecular and population stress responses in Daphnia magna exposed to cadmium. Environmental Science and Technology, 42(6), 2181-2188. https://doi.org/10.1021/es702469b

Linking molecular and population stress responses in Daphnia magna exposed to cadmium. / Connon, Richard E; Hooper, Helen L.; Sibly, Richard M.; Lim, Fei Ling; Heckmann, Lars Henrik; Moore, David J.; Watanabe, Hajime; Soetaert, Anneleen; Cook, Katie; Maund, Steve J.; Hutchinson, Thomas H.; Moggs, Jonathan; De Coen, Wim; Iguchi, Taisen; Callaghan, Amanda.

In: Environmental Science and Technology, Vol. 42, No. 6, 15.03.2008, p. 2181-2188.

Research output: Contribution to journalArticle

Connon, RE, Hooper, HL, Sibly, RM, Lim, FL, Heckmann, LH, Moore, DJ, Watanabe, H, Soetaert, A, Cook, K, Maund, SJ, Hutchinson, TH, Moggs, J, De Coen, W, Iguchi, T & Callaghan, A 2008, 'Linking molecular and population stress responses in Daphnia magna exposed to cadmium', Environmental Science and Technology, vol. 42, no. 6, pp. 2181-2188. https://doi.org/10.1021/es702469b
Connon, Richard E ; Hooper, Helen L. ; Sibly, Richard M. ; Lim, Fei Ling ; Heckmann, Lars Henrik ; Moore, David J. ; Watanabe, Hajime ; Soetaert, Anneleen ; Cook, Katie ; Maund, Steve J. ; Hutchinson, Thomas H. ; Moggs, Jonathan ; De Coen, Wim ; Iguchi, Taisen ; Callaghan, Amanda. / Linking molecular and population stress responses in Daphnia magna exposed to cadmium. In: Environmental Science and Technology. 2008 ; Vol. 42, No. 6. pp. 2181-2188.
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