Linking molecular and population stress responses in Daphnia magna exposed to cadmium

Richard E Connon, Helen L. Hooper, Richard M. Sibly, Fei Ling Lim, Lars Henrik Heckmann, David J. Moore, Hajime Watanabe, Anneleen Soetaert, Katie Cook, Steve J. Maund, Thomas H. Hutchinson, Jonathan Moggs, Wim De Coen, Taisen Iguchi, Amanda Callaghan

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

DNA microarrays can be used to measure environmental stress responses. If they are to be predictive of environmental impact, we need to determine if altered gene expression translates into negative impacts on individuals and populations. A large cDNA microarray (14000 spots) was created to measure molecular stress responses to cadmium in Daphnia magnate most widely used aquatic indicator species, and relate responses to population growth rate (pgr). We used the array to detect differences in the transcription of genes in juvenile D. magna (24 h old) after 24 h exposure to a control and three cadmium concentrations (6, 20, and 37 μg Cd2+ L-1). Stress responses at the population level were estimated following a further 8 days exposure. Pgr was approximately linear negative with increasing cadmium concentration over this range. The microarray profile of gene expression in response to acute cadmium exposure begins to provide an overview of the molecular responses of D. magna, especially in relation to growth and development. Of the responding genes, 29% were involved with metabolism including carbohydrate, fat and peptide metabolism, and energy production, 31% were involved with transcription/translation, while 40% of responding genes were associated with cellular processes like growth and moulting, ion transport, and general stress responses (which included oxidative stress). Our production and application of a large Daphnia magna microarray has shown that measured gene responses can be logically linked to the impact of a toxicant such as cadmium on somatic growth and development, and consequently pgr.

Original languageEnglish (US)
Pages (from-to)2181-2188
Number of pages8
JournalEnvironmental Science and Technology
Volume42
Issue number6
DOIs
StatePublished - Mar 15 2008

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Science(all)
  • Environmental Chemistry

Fingerprint Dive into the research topics of 'Linking molecular and population stress responses in Daphnia magna exposed to cadmium'. Together they form a unique fingerprint.

  • Cite this

    Connon, R. E., Hooper, H. L., Sibly, R. M., Lim, F. L., Heckmann, L. H., Moore, D. J., Watanabe, H., Soetaert, A., Cook, K., Maund, S. J., Hutchinson, T. H., Moggs, J., De Coen, W., Iguchi, T., & Callaghan, A. (2008). Linking molecular and population stress responses in Daphnia magna exposed to cadmium. Environmental Science and Technology, 42(6), 2181-2188. https://doi.org/10.1021/es702469b