Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling

Peta A. Neale, Selim Ait-Aissa, Werner Brack, Nicolas Creusot, Michael S. Denison, Björn Deutschmann, Klára Hilscherová, Henner Hollert, Martin Krauss, Jiří Novák, Tobias Schulze, Thomas Benjamin Seiler, Helene Serra, Ying Shao, Beate I. Escher

Research output: Contribution to journalArticle

96 Scopus citations

Abstract

Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effects of complex chemical mixtures. In this study, bioassays indicative of activation of the aryl hydrocarbon receptor (AhR), activation of the pregnane X receptor (PXR), activation of the estrogen receptor (ER), adaptive stress responses to oxidative stress (Nrf2), genotoxicity (p53) and inflammation (NF-κB) and the fish embryo toxicity test were applied along with chemical analysis to water extracts from the Danube River. Mixture-toxicity modeling was applied to determine the contribution of detected chemicals to the biological effect. Effect concentrations for between 0 to 13 detected chemicals could be found in the literature for the different bioassays. Detected chemicals explained less than 0.2% of the biological effect in the PXR activation, adaptive stress response, and fish embryo toxicity assays, while five chemicals explained up to 80% of ER activation, and three chemicals explained up to 71% of AhR activation. This study highlights the importance of fingerprinting the effects of detected chemicals.

Original languageEnglish (US)
Pages (from-to)14614-14624
Number of pages11
JournalEnvironmental Science and Technology
Volume49
Issue number24
DOIs
StatePublished - Dec 15 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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    Neale, P. A., Ait-Aissa, S., Brack, W., Creusot, N., Denison, M. S., Deutschmann, B., Hilscherová, K., Hollert, H., Krauss, M., Novák, J., Schulze, T., Seiler, T. B., Serra, H., Shao, Y., & Escher, B. I. (2015). Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling. Environmental Science and Technology, 49(24), 14614-14624. https://doi.org/10.1021/acs.est.5b04083