Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling

Peta A. Neale, Selim Ait-Aissa, Werner Brack, Nicolas Creusot, Michael S. Denison, Björn Deutschmann, Klára Hilscherová, Henner Hollert, Martin Krauss, Jiří Novák, Tobias Schulze, Thomas Benjamin Seiler, Helene Serra, Ying Shao, Beate I. Escher

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Surface water can contain countless organic micropollutants, and targeted chemical analysis alone may only detect a small fraction of the chemicals present. Consequently, bioanalytical tools can be applied complementary to chemical analysis to detect the effects of complex chemical mixtures. In this study, bioassays indicative of activation of the aryl hydrocarbon receptor (AhR), activation of the pregnane X receptor (PXR), activation of the estrogen receptor (ER), adaptive stress responses to oxidative stress (Nrf2), genotoxicity (p53) and inflammation (NF-κB) and the fish embryo toxicity test were applied along with chemical analysis to water extracts from the Danube River. Mixture-toxicity modeling was applied to determine the contribution of detected chemicals to the biological effect. Effect concentrations for between 0 to 13 detected chemicals could be found in the literature for the different bioassays. Detected chemicals explained less than 0.2% of the biological effect in the PXR activation, adaptive stress response, and fish embryo toxicity assays, while five chemicals explained up to 80% of ER activation, and three chemicals explained up to 71% of AhR activation. This study highlights the importance of fingerprinting the effects of detected chemicals.

Original languageEnglish (US)
Pages (from-to)14614-14624
Number of pages11
JournalEnvironmental Science and Technology
Volume49
Issue number24
DOIs
StatePublished - Dec 15 2015

Fingerprint

Surface waters
Toxicity
toxicity
surface water
Chemical activation
modeling
chemical analysis
Aryl Hydrocarbon Receptors
Bioassay
Estrogen Receptors
Fish
bioassay
embryo
Chemical analysis
hydrocarbon
Oxidative stress
micropollutant
effect
chemical
genotoxicity

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Neale, P. A., Ait-Aissa, S., Brack, W., Creusot, N., Denison, M. S., Deutschmann, B., ... Escher, B. I. (2015). Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling. Environmental Science and Technology, 49(24), 14614-14624. https://doi.org/10.1021/acs.est.5b04083

Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling. / Neale, Peta A.; Ait-Aissa, Selim; Brack, Werner; Creusot, Nicolas; Denison, Michael S.; Deutschmann, Björn; Hilscherová, Klára; Hollert, Henner; Krauss, Martin; Novák, Jiří; Schulze, Tobias; Seiler, Thomas Benjamin; Serra, Helene; Shao, Ying; Escher, Beate I.

In: Environmental Science and Technology, Vol. 49, No. 24, 15.12.2015, p. 14614-14624.

Research output: Contribution to journalArticle

Neale, PA, Ait-Aissa, S, Brack, W, Creusot, N, Denison, MS, Deutschmann, B, Hilscherová, K, Hollert, H, Krauss, M, Novák, J, Schulze, T, Seiler, TB, Serra, H, Shao, Y & Escher, BI 2015, 'Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling', Environmental Science and Technology, vol. 49, no. 24, pp. 14614-14624. https://doi.org/10.1021/acs.est.5b04083
Neale, Peta A. ; Ait-Aissa, Selim ; Brack, Werner ; Creusot, Nicolas ; Denison, Michael S. ; Deutschmann, Björn ; Hilscherová, Klára ; Hollert, Henner ; Krauss, Martin ; Novák, Jiří ; Schulze, Tobias ; Seiler, Thomas Benjamin ; Serra, Helene ; Shao, Ying ; Escher, Beate I. / Linking in Vitro Effects and Detected Organic Micropollutants in Surface Water Using Mixture-Toxicity Modeling. In: Environmental Science and Technology. 2015 ; Vol. 49, No. 24. pp. 14614-14624.
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