Abstract
Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-scale resolution challenge existing methods for CO identification. Taking advantage of linked-read sequencing, we develop a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first test this method using a pool of Arabidopsis F2 recombinants, and recapitulate results obtained from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from an F1 plant, we establish a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach enables the simultaneous generation and analysis of multiple CO landscapes, accelerating the pace at which mechanisms for the regulation of recombination can be elucidated through efficient comparisons of genotypic and environmental effects on recombination.
| Original language | English (US) |
|---|---|
| Number of pages | 1 |
| Journal | Nature communications |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 20 2019 |
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ASJC Scopus subject areas
- Chemistry(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Physics and Astronomy(all)
Cite this
Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination. / Sun, Hequan; Rowan, Beth A.; Flood, Pádraic J.; Brandt, Ronny; Fuss, Janina; Hancock, Angela M.; Michelmore, Richard W; Huettel, Bruno; Schneeberger, Korbinian.
In: Nature communications, Vol. 10, No. 1, 20.09.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Linked-read sequencing of gametes allows efficient genome-wide analysis of meiotic recombination
AU - Sun, Hequan
AU - Rowan, Beth A.
AU - Flood, Pádraic J.
AU - Brandt, Ronny
AU - Fuss, Janina
AU - Hancock, Angela M.
AU - Michelmore, Richard W
AU - Huettel, Bruno
AU - Schneeberger, Korbinian
PY - 2019/9/20
Y1 - 2019/9/20
N2 - Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-scale resolution challenge existing methods for CO identification. Taking advantage of linked-read sequencing, we develop a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first test this method using a pool of Arabidopsis F2 recombinants, and recapitulate results obtained from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from an F1 plant, we establish a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach enables the simultaneous generation and analysis of multiple CO landscapes, accelerating the pace at which mechanisms for the regulation of recombination can be elucidated through efficient comparisons of genotypic and environmental effects on recombination.
AB - Meiotic crossovers (COs) ensure proper chromosome segregation and redistribute the genetic variation that is transmitted to the next generation. Large populations and the demand for genome-wide, fine-scale resolution challenge existing methods for CO identification. Taking advantage of linked-read sequencing, we develop a highly efficient method for genome-wide identification of COs at kilobase resolution in pooled recombinants. We first test this method using a pool of Arabidopsis F2 recombinants, and recapitulate results obtained from the same plants using individual whole-genome sequencing. By applying this method to a pool of pollen DNA from an F1 plant, we establish a highly accurate CO landscape without generating or sequencing a single recombinant plant. The simplicity of this approach enables the simultaneous generation and analysis of multiple CO landscapes, accelerating the pace at which mechanisms for the regulation of recombination can be elucidated through efficient comparisons of genotypic and environmental effects on recombination.
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UR - http://www.scopus.com/inward/citedby.url?scp=85072538786&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-12209-2
DO - 10.1038/s41467-019-12209-2
M3 - Article
C2 - 31541084
AN - SCOPUS:85072538786
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
ER -