Lindane blocks GABA(A)-mediated inhibition and modulates pyramidal cell excitability in the rat hippocampal slice

R. M. Joy, W. F. Walby, L. G. Stark, Timothy E Albertson

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An in vitro paired-pulse orthodromic stimulation technique was used to examine the effects of lindane on excitatory afferent terminals, CA1 pyramidal cells and recurrent collateral evoked inhibition in the rat hippocampal slice. This was done to establish simultaneous effects on a simple neural network and to develop procedures for more detailed analyses of the effects of lindane. Hippocampal slices 400μm thick were perfused with oxygenated artificial cerebrospinal fluid. Electrodes were placed in the CA1 region to record extracellular population spike (PS) or excitatory postsynaptic potential (EPSP) responses to stimulation of Schaffer collateral/commissural (SC/C) fibers. Gamma-aminobutyric acid (GABA)mediated recurrent inhibition was measured using a paired-pulse technique. Perfusion with lindane produced both time and dose dependent changes in a number of the responses measured. The most striking effect produced by lindane was the loss of GABA(A)-mediated recurrent collateral inhibition. This tended to occur rapidly, often before changes in EPSP or PS responses could be detected. With longer exposures to lindane, repetitive discharge of pyramidal cells developed resulting in multiple PSs to single stimuli. Lindane (50 μM) also completely reversed the effects of the injectable anesthetic, propofol, a compound known to potentiate GABA(A)-mediated inhibition via a direct action on the GABA(A) receptor-chloride channel complex. An analysis of input/output relationships at varying stimulus intensities showed that lindane in creased EPSP and PS response amplitudes at any given stimulus intensity resulting in a leftward shift in the EPSP amplitude/stimulus intensity, PS amplitude/stimulus intensity and PS amplitude/EPSP amplitude relationships. This effect was most noticeable with low intensity stimuli and became progressively less so as stimulus intensities approached those yielding maximal responses. In addition lindane significantly increased paired pulse facilitation of EPSPs during paired stimulus presentation

Original languageEnglish (US)
Pages (from-to)217-228
Number of pages12
JournalNeuroToxicology
Volume16
Issue number2
StatePublished - 1995

Fingerprint

Lindane
Pyramidal Cells
gamma-Aminobutyric Acid
Rats
Excitatory Postsynaptic Potentials
Population
Cerebrospinal fluid
Chloride Channels
GABA Receptors
Propofol
Cerebrospinal Fluid
Anesthetics
Hippocampus
Electrodes
Perfusion
Neural networks
Injections
Fibers

Keywords

  • γ-Hexachlorocyclohexane
  • CA1 Pyramidal Cells
  • GABA
  • GABA(A)- Mediated Inhibition
  • Hippocampal Slice
  • Hippocampus
  • I/O Curves
  • Lindane
  • Organochlorine Insecticides
  • Paired Pulse Stimulation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience
  • Toxicology

Cite this

Lindane blocks GABA(A)-mediated inhibition and modulates pyramidal cell excitability in the rat hippocampal slice. / Joy, R. M.; Walby, W. F.; Stark, L. G.; Albertson, Timothy E.

In: NeuroToxicology, Vol. 16, No. 2, 1995, p. 217-228.

Research output: Contribution to journalArticle

@article{296408c9e0284cf4a4778b04a4a82631,
title = "Lindane blocks GABA(A)-mediated inhibition and modulates pyramidal cell excitability in the rat hippocampal slice",
abstract = "An in vitro paired-pulse orthodromic stimulation technique was used to examine the effects of lindane on excitatory afferent terminals, CA1 pyramidal cells and recurrent collateral evoked inhibition in the rat hippocampal slice. This was done to establish simultaneous effects on a simple neural network and to develop procedures for more detailed analyses of the effects of lindane. Hippocampal slices 400μm thick were perfused with oxygenated artificial cerebrospinal fluid. Electrodes were placed in the CA1 region to record extracellular population spike (PS) or excitatory postsynaptic potential (EPSP) responses to stimulation of Schaffer collateral/commissural (SC/C) fibers. Gamma-aminobutyric acid (GABA)mediated recurrent inhibition was measured using a paired-pulse technique. Perfusion with lindane produced both time and dose dependent changes in a number of the responses measured. The most striking effect produced by lindane was the loss of GABA(A)-mediated recurrent collateral inhibition. This tended to occur rapidly, often before changes in EPSP or PS responses could be detected. With longer exposures to lindane, repetitive discharge of pyramidal cells developed resulting in multiple PSs to single stimuli. Lindane (50 μM) also completely reversed the effects of the injectable anesthetic, propofol, a compound known to potentiate GABA(A)-mediated inhibition via a direct action on the GABA(A) receptor-chloride channel complex. An analysis of input/output relationships at varying stimulus intensities showed that lindane in creased EPSP and PS response amplitudes at any given stimulus intensity resulting in a leftward shift in the EPSP amplitude/stimulus intensity, PS amplitude/stimulus intensity and PS amplitude/EPSP amplitude relationships. This effect was most noticeable with low intensity stimuli and became progressively less so as stimulus intensities approached those yielding maximal responses. In addition lindane significantly increased paired pulse facilitation of EPSPs during paired stimulus presentation",
keywords = "γ-Hexachlorocyclohexane, CA1 Pyramidal Cells, GABA, GABA(A)- Mediated Inhibition, Hippocampal Slice, Hippocampus, I/O Curves, Lindane, Organochlorine Insecticides, Paired Pulse Stimulation",
author = "Joy, {R. M.} and Walby, {W. F.} and Stark, {L. G.} and Albertson, {Timothy E}",
year = "1995",
language = "English (US)",
volume = "16",
pages = "217--228",
journal = "NeuroToxicology",
issn = "0161-813X",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Lindane blocks GABA(A)-mediated inhibition and modulates pyramidal cell excitability in the rat hippocampal slice

AU - Joy, R. M.

AU - Walby, W. F.

AU - Stark, L. G.

AU - Albertson, Timothy E

PY - 1995

Y1 - 1995

N2 - An in vitro paired-pulse orthodromic stimulation technique was used to examine the effects of lindane on excitatory afferent terminals, CA1 pyramidal cells and recurrent collateral evoked inhibition in the rat hippocampal slice. This was done to establish simultaneous effects on a simple neural network and to develop procedures for more detailed analyses of the effects of lindane. Hippocampal slices 400μm thick were perfused with oxygenated artificial cerebrospinal fluid. Electrodes were placed in the CA1 region to record extracellular population spike (PS) or excitatory postsynaptic potential (EPSP) responses to stimulation of Schaffer collateral/commissural (SC/C) fibers. Gamma-aminobutyric acid (GABA)mediated recurrent inhibition was measured using a paired-pulse technique. Perfusion with lindane produced both time and dose dependent changes in a number of the responses measured. The most striking effect produced by lindane was the loss of GABA(A)-mediated recurrent collateral inhibition. This tended to occur rapidly, often before changes in EPSP or PS responses could be detected. With longer exposures to lindane, repetitive discharge of pyramidal cells developed resulting in multiple PSs to single stimuli. Lindane (50 μM) also completely reversed the effects of the injectable anesthetic, propofol, a compound known to potentiate GABA(A)-mediated inhibition via a direct action on the GABA(A) receptor-chloride channel complex. An analysis of input/output relationships at varying stimulus intensities showed that lindane in creased EPSP and PS response amplitudes at any given stimulus intensity resulting in a leftward shift in the EPSP amplitude/stimulus intensity, PS amplitude/stimulus intensity and PS amplitude/EPSP amplitude relationships. This effect was most noticeable with low intensity stimuli and became progressively less so as stimulus intensities approached those yielding maximal responses. In addition lindane significantly increased paired pulse facilitation of EPSPs during paired stimulus presentation

AB - An in vitro paired-pulse orthodromic stimulation technique was used to examine the effects of lindane on excitatory afferent terminals, CA1 pyramidal cells and recurrent collateral evoked inhibition in the rat hippocampal slice. This was done to establish simultaneous effects on a simple neural network and to develop procedures for more detailed analyses of the effects of lindane. Hippocampal slices 400μm thick were perfused with oxygenated artificial cerebrospinal fluid. Electrodes were placed in the CA1 region to record extracellular population spike (PS) or excitatory postsynaptic potential (EPSP) responses to stimulation of Schaffer collateral/commissural (SC/C) fibers. Gamma-aminobutyric acid (GABA)mediated recurrent inhibition was measured using a paired-pulse technique. Perfusion with lindane produced both time and dose dependent changes in a number of the responses measured. The most striking effect produced by lindane was the loss of GABA(A)-mediated recurrent collateral inhibition. This tended to occur rapidly, often before changes in EPSP or PS responses could be detected. With longer exposures to lindane, repetitive discharge of pyramidal cells developed resulting in multiple PSs to single stimuli. Lindane (50 μM) also completely reversed the effects of the injectable anesthetic, propofol, a compound known to potentiate GABA(A)-mediated inhibition via a direct action on the GABA(A) receptor-chloride channel complex. An analysis of input/output relationships at varying stimulus intensities showed that lindane in creased EPSP and PS response amplitudes at any given stimulus intensity resulting in a leftward shift in the EPSP amplitude/stimulus intensity, PS amplitude/stimulus intensity and PS amplitude/EPSP amplitude relationships. This effect was most noticeable with low intensity stimuli and became progressively less so as stimulus intensities approached those yielding maximal responses. In addition lindane significantly increased paired pulse facilitation of EPSPs during paired stimulus presentation

KW - γ-Hexachlorocyclohexane

KW - CA1 Pyramidal Cells

KW - GABA

KW - GABA(A)- Mediated Inhibition

KW - Hippocampal Slice

KW - Hippocampus

KW - I/O Curves

KW - Lindane

KW - Organochlorine Insecticides

KW - Paired Pulse Stimulation

UR - http://www.scopus.com/inward/record.url?scp=0028982366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028982366&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 217

EP - 228

JO - NeuroToxicology

JF - NeuroToxicology

SN - 0161-813X

IS - 2

ER -