The inter- and intramolecular interactions of lin-benzoadenine nucleotides have been examined by proton magnetic resonance. When the base is unprotonated, lin-benzoadenine nucleotides strongly stack in aqueous solution, with association constants of at least one order of magnitude greater than those of the corresponding adenine nucleotides. Some head-to-tail orientations of stacked lin-benzoadenine nucleotides were indicated by the deuterium substitution effect on relaxation times (DESERT). The relative positions of the heteroaromatic proton chemical shifts at infinite dilution (pD 8.5) and under acidic conditions (pD ∼4.0) indicated the conformations of the nucleotides (anti and syn, respectively) and the site of ring protonation (the pyrimidine ring).
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of the American Chemical Society|
|State||Published - 1979|
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