Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation

Laura Marcu, Amir Elbarbary, Patricia Zuk, Daniel A. De Ugarte, Prosper Benhaim, Hamza Kurt, Marc H. Hedrick, Peter Ashjian

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsS.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete
Pages250-257
Number of pages8
Volume4961
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV - San Jose, CA, United States
Duration: Jan 25 2003Jan 29 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV
CountryUnited States
CitySan Jose, CA
Period1/25/031/29/03

Keywords

  • Collagen
  • Time-resolved fluorescence spectroscopy
  • Tissue engineering osteogenesis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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