Abstract
Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | S.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete |
Pages | 250-257 |
Number of pages | 8 |
Volume | 4961 |
DOIs | |
State | Published - 2003 |
Event | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV - San Jose, CA, United States Duration: Jan 25 2003 → Jan 29 2003 |
Other
Other | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV |
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Country | United States |
City | San Jose, CA |
Period | 1/25/03 → 1/29/03 |
Keywords
- Collagen
- Time-resolved fluorescence spectroscopy
- Tissue engineering osteogenesis
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics