Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation

Laura Marcu, Amir Elbarbary, Patricia Zuk, Daniel A. De Ugarte, Prosper Benhaim, Hamza Kurt, Marc H. Hedrick, Peter Ashjian

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsS.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete
Pages250-257
Number of pages8
Volume4961
DOIs
StatePublished - 2003
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV - San Jose, CA, United States
Duration: Jan 25 2003Jan 29 2003

Other

OtherPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV
CountryUnited States
CitySan Jose, CA
Period1/25/031/29/03

Fingerprint

Fluorescence spectroscopy
Collagen
collagens
life (durability)
fluorescence
Fluorescence
laser induced fluorescence
Lasers
spectroscopy
Tissue
connective tissue
nitrogen lasers
Stem cells
stem cells
pulsed lasers
Nitrogen
Monitoring
Chemical analysis
matrices

Keywords

  • Collagen
  • Time-resolved fluorescence spectroscopy
  • Tissue engineering osteogenesis

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Marcu, L., Elbarbary, A., Zuk, P., De Ugarte, D. A., Benhaim, P., Kurt, H., ... Ashjian, P. (2003). Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation. In S. L. Jacques, D. D. Duncan, S. J. Kirkpatrick, & A. Kriete (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4961, pp. 250-257) https://doi.org/10.1117/12.477913

Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation. / Marcu, Laura; Elbarbary, Amir; Zuk, Patricia; De Ugarte, Daniel A.; Benhaim, Prosper; Kurt, Hamza; Hedrick, Marc H.; Ashjian, Peter.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / S.L. Jacques; D.D. Duncan; S.J. Kirkpatrick; A. Kriete. Vol. 4961 2003. p. 250-257.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Marcu, L, Elbarbary, A, Zuk, P, De Ugarte, DA, Benhaim, P, Kurt, H, Hedrick, MH & Ashjian, P 2003, Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation. in SL Jacques, DD Duncan, SJ Kirkpatrick & A Kriete (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4961, pp. 250-257, PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Laser-Tissue Interaction XIV, San Jose, CA, United States, 1/25/03. https://doi.org/10.1117/12.477913
Marcu L, Elbarbary A, Zuk P, De Ugarte DA, Benhaim P, Kurt H et al. Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation. In Jacques SL, Duncan DD, Kirkpatrick SJ, Kriete A, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 4961. 2003. p. 250-257 https://doi.org/10.1117/12.477913
Marcu, Laura ; Elbarbary, Amir ; Zuk, Patricia ; De Ugarte, Daniel A. ; Benhaim, Prosper ; Kurt, Hamza ; Hedrick, Marc H. ; Ashjian, Peter. / Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation. Proceedings of SPIE - The International Society for Optical Engineering. editor / S.L. Jacques ; D.D. Duncan ; S.J. Kirkpatrick ; A. Kriete. Vol. 4961 2003. pp. 250-257
@inproceedings{84bdaa24a5b84372b0d0ffdece33620e,
title = "Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation",
abstract = "Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.",
keywords = "Collagen, Time-resolved fluorescence spectroscopy, Tissue engineering osteogenesis",
author = "Laura Marcu and Amir Elbarbary and Patricia Zuk and {De Ugarte}, {Daniel A.} and Prosper Benhaim and Hamza Kurt and Hedrick, {Marc H.} and Peter Ashjian",
year = "2003",
doi = "10.1117/12.477913",
language = "English (US)",
volume = "4961",
pages = "250--257",
editor = "S.L. Jacques and D.D. Duncan and S.J. Kirkpatrick and A. Kriete",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

}

TY - GEN

T1 - Lifetime fluorescence spectroscopy for in-situ investigation of osteogenic differentiation

AU - Marcu, Laura

AU - Elbarbary, Amir

AU - Zuk, Patricia

AU - De Ugarte, Daniel A.

AU - Benhaim, Prosper

AU - Kurt, Hamza

AU - Hedrick, Marc H.

AU - Ashjian, Peter

PY - 2003

Y1 - 2003

N2 - Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.

AB - Time-Resolved Laser-Induced Fluorescence Spectroscopy (TR-LIFS) represents a potential tool for the in-situ characterization of bioengineered tissues. In this study, we evaluate the application of TR-LIFS to non-intrusive monitoring of matrix composition during osteogenetic differentiation. Human adipose-derived stem cells, harvested from 3 patients, were induced in osteogenic media for 3, 5, and 7 weeks. Samples were subsequently collected and probed for time-resolved fluorescence emission with a pulsed nitrogen laser. Fluorescence parameters, derived from both spectral- and time-domain, were used for sample characterization. The samples were further analyzed using Western blot analysis and computer-based densitometry. A significant change in the fluorescence parameters was detected for samples beyond 3 weeks of osteogenic differentiation. The spectroscopic observations: 1) show increase of collagen I when contrasted against the time-resolved fluorescence spectra of commercially available collagens; and 2) are in agreement with Western blot analysis that demonstrated significant increase in collagen I content between 3- vs. 5-weeks and 3- vs. 7-weeks and no changes for collagens III, IV, and V. Our results suggest that TR-LIFS can be used as a non-invasive means for the detection of specific collagens in maturing connective tissues.

KW - Collagen

KW - Time-resolved fluorescence spectroscopy

KW - Tissue engineering osteogenesis

UR - http://www.scopus.com/inward/record.url?scp=0345714665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345714665&partnerID=8YFLogxK

U2 - 10.1117/12.477913

DO - 10.1117/12.477913

M3 - Conference contribution

AN - SCOPUS:0345714665

VL - 4961

SP - 250

EP - 257

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Jacques, S.L.

A2 - Duncan, D.D.

A2 - Kirkpatrick, S.J.

A2 - Kriete, A.

ER -