Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation

Neha Dixit, Min Ho Kim, Jan Rossaint, Itsukyo Yamayoshi, Alexander Zarbock, Scott I. Simon

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Neutrophil arrest and migration on inflamed endothelium involves a conformational shift in CD11a/CD18 (leukocyte function antigen-1; LFA-1) to a high-affinity and clustered state that determines the strength and lifetime of bond formation with ICAM-1. Cytoskeletal adapter proteins Kindlin-3 and Talin-1 anchor clustered LFA-1 to the cytoskeleton and facilitate the transition from neutrophil rolling to arrest. We recently reported that tensile force acts on LFA-1 bonds inducing their colocalization with Orai1, the predominant membrane store operated Ca2+ channel that cooperates with the endoplasmic reticulum to elicit cytosolic flux. Because Kindlin-3 was recently reported to initiate LFA-1 clustering in lymphocytes, we hypothesized that it cooperates with Orai1 and LFA-1 in signaling local Ca2+ flux necessary for shear-resistant neutrophil arrest. Using microfluidic flow channels combined with total internal reflection fluorescence microscopy, we applied defined shear stress to low- or high-affinity LFA-1 and imaged the spatiotemporal regulation of bond formation with Kindlin-3 recruitment and Ca2+ influx. Orai1 and Kindlin-3 genes were silenced in neutrophil-like HL-60 cells to assess their respective roles in this process. Kindlin-3 was enriched within focal clusters of high-affinity LFA-1, which promoted physical linkage with Orai1. This macromolecular complex functioned to amplify inside-out Ca2+ signaling in response to IL-8 stimulation by catalyzing an increased density of Talin-1 and consolidating LFA-1 clusters within sites of contact with ICAM-1. In this manner, neutrophils use focal adhesions as mechanosensors that convert shear stress-mediated tensile force into local bursts of Ca2+ influx that catalyze cytoskeletal engagement and an adhesion-strengthened migratory phenotype.

Original languageEnglish (US)
Pages (from-to)5954-5964
Number of pages11
JournalJournal of Immunology
Volume189
Issue number12
DOIs
StatePublished - Dec 15 2012

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Lymphocyte Function-Associated Antigen-1
Neutrophil Infiltration
HLA Antigens
Inflammation
Calcium
Neutrophils
Talin
Intercellular Adhesion Molecule-1
Macromolecular Substances
Focal Adhesions
Cytoskeletal Proteins
Microfluidics
HL-60 Cells
Cytoskeleton
Interleukin-8
Fluorescence Microscopy
Endoplasmic Reticulum
Endothelium
Cluster Analysis
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation. / Dixit, Neha; Kim, Min Ho; Rossaint, Jan; Yamayoshi, Itsukyo; Zarbock, Alexander; Simon, Scott I.

In: Journal of Immunology, Vol. 189, No. 12, 15.12.2012, p. 5954-5964.

Research output: Contribution to journalArticle

Dixit, Neha ; Kim, Min Ho ; Rossaint, Jan ; Yamayoshi, Itsukyo ; Zarbock, Alexander ; Simon, Scott I. / Leukocyte function antigen-1, kindlin-3, and calcium flux orchestrate neutrophil recruitment during inflammation. In: Journal of Immunology. 2012 ; Vol. 189, No. 12. pp. 5954-5964.
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