Leukemia inhibitory factor and ciliary neurotrophic factor cause dendritic retraction in cultured rat sympathetic neurons

Xin Quo, Vidya Chandrasekaran, Pamela J Lein, Paul L. Kaplan, Dennis Higgins

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Dendritic retraction occurs in many regions of the developing brain and also after neural injury. However, the molecules that regulate this important regressive process remain largely unknown. Our data indicate that leukemia inhibitory factor (LIF) and ciliary neurotrophic factor (CNTF) cause sympathetic neurons to retract their dendrites in vitro, ultimately leading to an ~80% reduction in the size of the arbor. The dendritic retraction induced by LIF exhibited substantial specificity because it was not accompanied by changes in cell number, in the rate of axonal growth, or in the expression of axonal cytoskeletal elements. An antibody to gp130 blocked the effects of LIF and CNTF, and both cytokines induced phosphorylation and nuclear translocation of stat3. Moreover, addition of soluble interleukin-6 (IL-6) receptor to the medium endowed IL-6 with the ability to cause dendritic regression. These data indicate that ligands activating the gp130 pathway have the ability to profoundly alter neuronal cell shape and polarity by selectively causing the retraction of dendrites.

Original languageEnglish (US)
Pages (from-to)2113-2121
Number of pages9
JournalJournal of Neuroscience
Volume19
Issue number6
StatePublished - Mar 15 1999
Externally publishedYes

Fingerprint

Ciliary Neurotrophic Factor
Leukemia Inhibitory Factor
Dendrites
Neurons
Interleukin-6 Receptors
Cell Polarity
Cell Shape
Interleukin-6
Cell Count
Phosphorylation
Cytokines
Ligands
Antibodies
Wounds and Injuries
Brain
Growth

Keywords

  • Bone morphogenetic protein
  • Ciliary neurotrophic factor
  • Dendrites
  • Dendritic retraction
  • Gp130
  • Leukemia inhibitory factor
  • Osteogenic protein-1
  • Stat3
  • Sympathetic neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Leukemia inhibitory factor and ciliary neurotrophic factor cause dendritic retraction in cultured rat sympathetic neurons. / Quo, Xin; Chandrasekaran, Vidya; Lein, Pamela J; Kaplan, Paul L.; Higgins, Dennis.

In: Journal of Neuroscience, Vol. 19, No. 6, 15.03.1999, p. 2113-2121.

Research output: Contribution to journalArticle

Quo, Xin ; Chandrasekaran, Vidya ; Lein, Pamela J ; Kaplan, Paul L. ; Higgins, Dennis. / Leukemia inhibitory factor and ciliary neurotrophic factor cause dendritic retraction in cultured rat sympathetic neurons. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 6. pp. 2113-2121.
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