Lethal effects of Clostridium perfringens epsilon toxin are potentiated by alpha and perfringolysin-O toxins in a mouse model

Mariano E. Fernandez-Miyakawa, B. Helen Jost, Stephen J. Billington, Francisco A Uzal

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Epsilon toxin (ETX) is the most important virulence factor of Clostridium perfringens type D. Two other important toxins, alpha toxin (CPA) and perfringolysin-O (PFO), are encoded and potentially produced by most C. perfringens type D isolates. The biological effects of these toxins are dissimilar although they are all lethal. Since the possible interaction of these toxins during infection is unknown, the effects of CPA and PFO on the lethal activity of ETX were studied in a mouse model. Mice were injected intravenously or intragastrically with CPA or PFO with or without ETX. Sublethal doses of CPA or PFO did not affect the lethality of ETX when either was injected together with the latter intravenously. However, sublethal or lethal doses of CPA or PFO resulted in reduction of the survival time of mice injected simultaneously with ETX when compared with the intravenous effect of ETX injected alone. When PFO was inoculated intragastrically with ETX, a reduction of the survival time was observed. CPA did not alter the survival time when inoculated intragastrically with ETX. The results of the present study suggest that both CPA and PFO have the potential to enhance the ETX lethal effects during enterotoxemia in natural hosts such as sheep and goats.

Original languageEnglish (US)
Pages (from-to)379-385
Number of pages7
JournalVeterinary Microbiology
Volume127
Issue number3-4
DOIs
StatePublished - Mar 18 2008

Keywords

  • Alpha toxin
  • Clostridium perfringens
  • Epsilon toxin
  • Perfringolysin-O
  • Synergism

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Lethal effects of Clostridium perfringens epsilon toxin are potentiated by alpha and perfringolysin-O toxins in a mouse model'. Together they form a unique fingerprint.

  • Cite this