Abstract
Cortical pulling on astral microtubules positions the mitotic spindle in response to PAR polarity cues and G protein signaling in many systems. In Caenorhabditis elegans single-cell embryos, posterior spindle displacement depends on Gα and its regulators GPR-1/2 and LIN-5. GPR-1/2 and LIN-5 are necessary for cortical pulling forces and become enriched at the posterior cortex, which suggests that higher forces act on the posterior spindle pole compared with the anterior pole. However, the precise distribution of cortical forces and how they are regulated remains to be determined. Using spindle severing, single centrosome assays, and centrosome fragmentation, we show that both the anterior and posterior cortices generate more pulling force than the lateral-posterior region. Lateral inhibition depends on LET-99, which inhibits GPR-1/2 localization to produce a bipolar GPR-1/2 pattern. Thus, rather than two domains of cortical force, there are three. We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 481-495 |
| Number of pages | 15 |
| Journal | Journal of Cell Biology |
| Volume | 189 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 3 2010 |
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ASJC Scopus subject areas
- Cell Biology
Cite this
LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos. / Krueger, Lori E.; Wu, Jui Ching; Tsou, Meng Fu Bryan; Rose, Lesilee S.
In: Journal of Cell Biology, Vol. 189, No. 3, 03.05.2010, p. 481-495.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos
AU - Krueger, Lori E.
AU - Wu, Jui Ching
AU - Tsou, Meng Fu Bryan
AU - Rose, Lesilee S.
PY - 2010/5/3
Y1 - 2010/5/3
N2 - Cortical pulling on astral microtubules positions the mitotic spindle in response to PAR polarity cues and G protein signaling in many systems. In Caenorhabditis elegans single-cell embryos, posterior spindle displacement depends on Gα and its regulators GPR-1/2 and LIN-5. GPR-1/2 and LIN-5 are necessary for cortical pulling forces and become enriched at the posterior cortex, which suggests that higher forces act on the posterior spindle pole compared with the anterior pole. However, the precise distribution of cortical forces and how they are regulated remains to be determined. Using spindle severing, single centrosome assays, and centrosome fragmentation, we show that both the anterior and posterior cortices generate more pulling force than the lateral-posterior region. Lateral inhibition depends on LET-99, which inhibits GPR-1/2 localization to produce a bipolar GPR-1/2 pattern. Thus, rather than two domains of cortical force, there are three. We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement.
AB - Cortical pulling on astral microtubules positions the mitotic spindle in response to PAR polarity cues and G protein signaling in many systems. In Caenorhabditis elegans single-cell embryos, posterior spindle displacement depends on Gα and its regulators GPR-1/2 and LIN-5. GPR-1/2 and LIN-5 are necessary for cortical pulling forces and become enriched at the posterior cortex, which suggests that higher forces act on the posterior spindle pole compared with the anterior pole. However, the precise distribution of cortical forces and how they are regulated remains to be determined. Using spindle severing, single centrosome assays, and centrosome fragmentation, we show that both the anterior and posterior cortices generate more pulling force than the lateral-posterior region. Lateral inhibition depends on LET-99, which inhibits GPR-1/2 localization to produce a bipolar GPR-1/2 pattern. Thus, rather than two domains of cortical force, there are three. We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement.
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UR - http://www.scopus.com/inward/citedby.url?scp=77951823707&partnerID=8YFLogxK
U2 - 10.1083/jcb.201001115
DO - 10.1083/jcb.201001115
M3 - Article
C2 - 20421425
AN - SCOPUS:77951823707
VL - 189
SP - 481
EP - 495
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 3
ER -