LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos

Lori E. Krueger, Jui Ching Wu, Meng Fu Bryan Tsou, Lesilee S. Rose

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Cortical pulling on astral microtubules positions the mitotic spindle in response to PAR polarity cues and G protein signaling in many systems. In Caenorhabditis elegans single-cell embryos, posterior spindle displacement depends on Gα and its regulators GPR-1/2 and LIN-5. GPR-1/2 and LIN-5 are necessary for cortical pulling forces and become enriched at the posterior cortex, which suggests that higher forces act on the posterior spindle pole compared with the anterior pole. However, the precise distribution of cortical forces and how they are regulated remains to be determined. Using spindle severing, single centrosome assays, and centrosome fragmentation, we show that both the anterior and posterior cortices generate more pulling force than the lateral-posterior region. Lateral inhibition depends on LET-99, which inhibits GPR-1/2 localization to produce a bipolar GPR-1/2 pattern. Thus, rather than two domains of cortical force, there are three. We propose that the attenuation of lateral forces prevents counterproductive pulling, resulting in a higher net force toward the posterior that contributes to spindle elongation and displacement.

Original languageEnglish (US)
Pages (from-to)481-495
Number of pages15
JournalJournal of Cell Biology
Volume189
Issue number3
DOIs
StatePublished - May 3 2010

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Linear Energy Transfer
Centrosome
Embryonic Structures
Spindle Poles
Spindle Apparatus
Caenorhabditis elegans
GTP-Binding Proteins
Microtubules
Cues
Inhibition (Psychology)

ASJC Scopus subject areas

  • Cell Biology

Cite this

LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos. / Krueger, Lori E.; Wu, Jui Ching; Tsou, Meng Fu Bryan; Rose, Lesilee S.

In: Journal of Cell Biology, Vol. 189, No. 3, 03.05.2010, p. 481-495.

Research output: Contribution to journalArticle

Krueger, Lori E. ; Wu, Jui Ching ; Tsou, Meng Fu Bryan ; Rose, Lesilee S. / LET-99 inhibits lateral posterior pulling forces during asymmetric spindle elongation in C. elegans embryos. In: Journal of Cell Biology. 2010 ; Vol. 189, No. 3. pp. 481-495.
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