Lesions of the enamel organ of developing dog teeth following experimental inoculation of gnotobiotic puppies with canine distemper virus

R. R. Dubielzig, Robert Higgins, S. Krakowka

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Ten 7-day-old gnotobiotic Beagle puppies were inoculated intraperitoneally with virulent canine distemper virus (R-252-CDV). The dogs were killed and perfused with paraformaldehyde/glutaraldehyde from eight to 36 days after inoculation. The developing teeth of the mandibles were examined by light microscopy, and the teeth from three dogs were examined by electron microscopy. Necrosis of individual cells in the stratum intermedium of the developing tooth was the first change, detectable at day 9 post-inoculation. At day 16 post-inoculation, there was disorganization of the ameloblasts. In the stratum intermedium, multinucleate giant cells and large eosinophilic cytoplasmic viral inclusions were prominent. Ultrastructurally, these inclusions consisted of clusters of tubular aggregates typical of canine distemper virus nucleocapsids. At 28 to 36 days post-inoculation, the changes were seen in the reduced enamel epithelium. Multinucleate cells were seen, but no inclusions. Some necrotic cells were seen. In these teeth, ameloblastic cells of the root were morphologically normal. Our results suggest that distemper virus affects developing teeth by direct infection of the enamel organ.

Original languageEnglish (US)
Pages (from-to)684-689
Number of pages6
JournalVeterinary Pathology
Volume18
Issue number5
DOIs
StatePublished - Jan 1 1981
Externally publishedYes

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Enamel Organ
Canine Distemper Virus
Germ-Free Life
Canine distemper virus
enamel
puppies
lesions (animal)
Tooth
teeth
Dogs
dogs
Distemper
cells
Ameloblasts
distemper
Nucleocapsid
nucleocapsid
Inclusion Bodies
glutaraldehyde
Glutaral

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lesions of the enamel organ of developing dog teeth following experimental inoculation of gnotobiotic puppies with canine distemper virus. / Dubielzig, R. R.; Higgins, Robert; Krakowka, S.

In: Veterinary Pathology, Vol. 18, No. 5, 01.01.1981, p. 684-689.

Research output: Contribution to journalArticle

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