Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat

Anne A Knowlton, Carl S. Apstein, Rola Saouf, Peter Brecher

Research output: Contribution to journalArticle

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Abstract

Fatty acids and their metabolites have been implicated as a cause of myocardial damage during ischemia. Fatty acid binding protein (FABP), an abundant low molecular weight protein present in the cytosol of myocytes, has been postulated to be a key fatty acid carrier protein in the myocardium. Postulating that loss of FABP during ischemia could cause an increase in unbound intracellular fatty acids contributing to myocardial damage, we measured plasma FABP levels during 60 min of myocardial ischemia followed by 60 min of reperfusion in the rat. Peak levels were seen 15 min after reperfusion. Plasma levels were higher with larger areas of myocardial ischemia (1720 ± 528 ng/ml vs. 216 ± 76 ng/ml with smaller areas, P < 0.02). Tissue levels after 60 min of ischemia and 60 min of reperfusion were decreased by over 50%, (1.0 ± 0.3 mg FABP/g wet wt compared with 2.9 ± 0.4 mg FABP/g wet wt in normal myocardium, P < 0.005). The data is consistent with the proposal that loss of FABP contributes to the myocardial damage associated with ischemia and reperfusion. Additional studies are needed to determine the exact role of FABP in the regulation of fatty acid metabolism in the heart.

Original languageEnglish (US)
Pages (from-to)577-583
Number of pages7
JournalJournal of Molecular and Cellular Cardiology
Volume21
Issue number6
DOIs
StatePublished - 1989
Externally publishedYes

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Keywords

  • Fatty acid binding protein
  • Myocardial ischemia
  • Myocardial metabolism
  • Reperfusion injury

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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