Lead exposure delays the differentiation of oligodendroglial progenitors in vitro

Wenbin Deng, Randall D. McKinnon, Ronald D. Poretz

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Lead (Pb) is an environmental neurotoxicant that can cause hypo-and demyelination. Oligodendrocytes (OLs), the myelin-forming cells in the central nervous system, may be a possible target for Pb toxicity. The present study describes the effect of Pb on the maturation of rat OL progenitor (OP) cells and the developmental expression of myelin-specific galactolipids. Dose - response studies showed that OP cultures were more sensitive to Pb than mature OLs. Pb delayed the differentiation of OL progenitors, as demonstrated by cell morphology and immunostaining With a panel of stage-specific differentiation markers. Pb given prior to and during differentiation caused a decrease in the biosynthesis of galactolipids in both undifferentiated and differentiated OLs, as detected by metabolic radiolabeling with 3H-D-galactose. While the ratios of galacto/gluco-cerebrosides, hydroxy fatty acid/nonhydroxy fatty acid galactolipids, and galactocerebrosides/ sulfatides increased in control cultures during cell differentiation, Pb treatment prevented these changes. The results suggest that chronic Pb exposure may impact brain development by interfering with the timely developmental maturation of OL progenitors.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalToxicology and Applied Pharmacology
Volume174
Issue number3
DOIs
StatePublished - Aug 1 2001
Externally publishedYes

Keywords

  • Differentiation
  • Galactolipid
  • Lead toxicity
  • Myelination
  • Oligodendrocyte

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

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