LC-MS/MS screen for penitrem A and roquefortine C in serum and urine samples

Elizabeth R. Tor, Birgit Puschner, Michael S. Filigenzi, Asheesh K. Tiwary, Robert H Poppenga

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

A rapid LC-MS/MS method, using a triple quadrupole/linear ion trap mass spectrometer, was developed for determination of penitrem A and roquefortine C in serum and urine samples. Penitrem A and roquefortine C were extracted from samples with methylene chloride. The extracts were injected onto a liquid Chromatograph coupled with a hybrid triple quadrupole/linear ion trap mass spectrometer. Seven replicate fortifications of serum at 0.001 μg/g (1 ppb) each of penitrem A and roquefortine C gave average recoveries of 90% with 10% CV (relative standard deviation) and 97% with 3% CV, respectively. Seven replicate fortifications of urine at 0.001 μg/g (1 ppb) each of penitrem A and roquefortine C gave average recoveries of 98% with 12% CV and 100% with 6% CV, respectively. This is the first report of a positive mass spectrometric identification and quantitation of both compounds in urine and serum samples from dog intoxication cases.

Original languageEnglish (US)
Pages (from-to)4624-4629
Number of pages6
JournalAnalytical Chemistry
Volume78
Issue number13
DOIs
StatePublished - Jul 1 2006

ASJC Scopus subject areas

  • Analytical Chemistry

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