Latent Murine Cytomegalovirus DNA in Splenic Stromal Cells of Mice

Claire Pomeroy, Patricia J. Hilleren, M. Colin Jordan

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Latency is an integral feature of the pathogenesis of cytomegalovirus infection and disease. Using in situ hybridization, we detected viral DNA in the splenic stroma of mice with acute murine cytomegalovirus (MCMV) infection but could not detect latent infection. By using enzymatic amplification of a 700-bp region of exon 4 of immediate-early gene 1 of MCMV, viral DNA was consistently detected in whole spleens of latently infected mice. MCMV DNA was detected in 16 of 23 stromal cell fractions from latently infected animals, in only 2 of 13 residual nonstromal cell fractions, and in none of 9 additional lymphocyte or macrophage-enriched nonstromal cell preparations. We conclude that MCMV DNA is maintained predominantly, and possibly exclusively, in stromal cells in the spleens of latently infected mice.

Original languageEnglish (US)
Pages (from-to)3330-3334
Number of pages5
JournalJournal of Virology
Volume65
Issue number6
StatePublished - Jun 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Pomeroy, C., Hilleren, P. J., & Colin Jordan, M. (1991). Latent Murine Cytomegalovirus DNA in Splenic Stromal Cells of Mice. Journal of Virology, 65(6), 3330-3334.