Late-gestation ventricular myocardial reduction in fetuses of hyperglycemic CD1 mice is associated with increased apoptosis

Juan claudio Gutierrez, M. Renee Prater, Bonnie J. Smith, Larry E. Freeman, Murali K. Mallela, Steven D. Holladay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

BACKGROUND: Previous work in our laboratory showed reduced myocardium and dilated ventricular chambers in gestation day (GD) 17 hearts that were collected from hyperglycemic CD1 mouse dams. Pre-breeding maternal immune stimulation, using Freund's complete adjuvant (FCA), diminished the severity of these fetal heart lesions. The following experiments were performed to detect possible changes in fetal heart apoptotic cell death, under hyperglycemic conditions and with or without maternal immune stimulation. METHODS: Female CD1 mice were injected with 200 mg/kg of streptozocin (STZ) to induce insulin-dependent diabetes mellitus. Half of these mice received prior FCA injection. Fetal hearts were collected on GD 17 and myocardial apoptotic cells were quantified using flow cytometry. A panel of apoptosis regulatory genes (Bcl2, p53, Casp3, Casp9, PkCe) was then examined in the fetal myocardium using RT-PCR. RESULTS: Early apoptotic cells and late apoptotic/necrotic cells were significantly increased in fetal hearts from STZ or STZ+FCA dams. Pre-treatment with FCA reduced late apoptotic/necrotic cells to control level, suggesting some cell death protection was rendered by FCA. Paradoxically in the face of such increased cell death, the expression of pro-apoptotic genes Casp3 and Casp9 was decreased by diabetes, while the anti-apoptotic gene Bcl2 was increased. CONCLUSIONS: Maternal hyperglycemia causes dys-regulated apoptosis of fetal myocardial cells. Such effect may be prevented by maternal immune stimulation.

Original languageEnglish (US)
Pages (from-to)409-415
Number of pages7
JournalBirth Defects Research Part B - Developmental and Reproductive Toxicology
Volume86
Issue number5
DOIs
StatePublished - Oct 2009
Externally publishedYes

Fingerprint

Obese Mice
Freund's Adjuvant
Fetal Heart
Fetus
Apoptosis
Cell death
Pregnancy
Streptozocin
Mothers
Cell Death
Genes
Medical problems
Dams
Myocardium
Cells
Cytoprotection
Flow cytometry
Level control
Regulator Genes
Type 1 Diabetes Mellitus

Keywords

  • Apoptosis
  • Bcl2
  • Diabetes
  • Fetal heart
  • Hyperglycemia
  • Mouse

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Late-gestation ventricular myocardial reduction in fetuses of hyperglycemic CD1 mice is associated with increased apoptosis. / Gutierrez, Juan claudio; Prater, M. Renee; Smith, Bonnie J.; Freeman, Larry E.; Mallela, Murali K.; Holladay, Steven D.

In: Birth Defects Research Part B - Developmental and Reproductive Toxicology, Vol. 86, No. 5, 10.2009, p. 409-415.

Research output: Contribution to journalArticle

Gutierrez, Juan claudio ; Prater, M. Renee ; Smith, Bonnie J. ; Freeman, Larry E. ; Mallela, Murali K. ; Holladay, Steven D. / Late-gestation ventricular myocardial reduction in fetuses of hyperglycemic CD1 mice is associated with increased apoptosis. In: Birth Defects Research Part B - Developmental and Reproductive Toxicology. 2009 ; Vol. 86, No. 5. pp. 409-415.
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