Laser welding and collagen crosslinks

Karen M. Reiser, Ward Small IV, Duncan J. Maitland, Nicholas J. Heredia, Luiz B. Da Silva, Dennis L Matthews, Jerold A Last

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The strength and stability of laser-welded tissue may be influenced, in part, by the effects of laser exposure on collagen crosslinking. We therefore studied the effects of diode laser exposure (805 nm, 1 - 8 watts, 30 seconds) plus indocyanine green dye (ICG) on calf tail tendon collagen crosslinks. The effect of ICG dye alone on crosslink content prior to laser exposure was investigated; unexpectedly, we found that ICG-treated tissue had significantly increased DHLNL and OHP, but not HLNL. Laser exposure after ICG application reduced elevated DHLNL and OHP crosslink content down to their native levels. The monohydroxylated crosslink HLNL was inversely correlated with laser output (p less than 0.01 by linear regression analysis). DHLNL content was highly correlated with content of its maturational product, OHP, suggesting that precursor-product relationships are maintained. We conclude that: (1) ICG alone induces DHLNL and OHP crosslink formation; (2) subsequent laser exposure reduces the ICG-induced crosslinks down to native levels; (3) excessive diode laser exposure destroys normally occurring HLNL crosslinks.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Number of pages4
StatePublished - 1997
EventLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII - San Jose, CA, United States
Duration: Feb 8 1997Feb 8 1997


OtherLasers in Surgery: Advanced Characterization, Therapeutics, and Systems VII
Country/TerritoryUnited States
CitySan Jose, CA

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics


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