Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells

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17 Citations (Scopus)

Abstract

PURPOSE. To investigate the cellular mechanisms involved in the cell death of human retinal pigment epithelial (hRPE) cells after their exposure to laser injury. METHODS. Cultured human hRPE cells were irradiated for different lengths of time and at different levels of energy using diode laser photocoagulation coupled with an intraocular laser probe. Apoptosis was determined by TUNEL staining and annexin-V labeling of phosphatidylserine exposure. Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatography. RESULTS. Laser irradiation caused areas of apoptosis in the hRPE cells. These areas were detected around the ablated and necrotic laser scar and developed several hours after the laser irradiation. Laser irradiation concomitantly induced an increase in the intracellular production of ceramide, a lipid second messenger. CONCLUSIONS. The results demonstrate that laser irradiation induces apoptosis in hRPE cells and suggest that the underlying signaling mechanism involves ceramide generation.

Original languageEnglish (US)
Pages (from-to)2587-2591
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume46
Issue number7
DOIs
StatePublished - 2005

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Retinal Pigments
Ceramides
Lasers
Epithelial Cells
Apoptosis
Diacylglycerol Kinase
Semiconductor Lasers
Light Coagulation
Annexin A5
Phosphatidylserines
In Situ Nick-End Labeling
Second Messenger Systems
Thin Layer Chromatography
Cicatrix
Cell Death
Staining and Labeling
Lipids
Wounds and Injuries

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells",
abstract = "PURPOSE. To investigate the cellular mechanisms involved in the cell death of human retinal pigment epithelial (hRPE) cells after their exposure to laser injury. METHODS. Cultured human hRPE cells were irradiated for different lengths of time and at different levels of energy using diode laser photocoagulation coupled with an intraocular laser probe. Apoptosis was determined by TUNEL staining and annexin-V labeling of phosphatidylserine exposure. Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatography. RESULTS. Laser irradiation caused areas of apoptosis in the hRPE cells. These areas were detected around the ablated and necrotic laser scar and developed several hours after the laser irradiation. Laser irradiation concomitantly induced an increase in the intracellular production of ceramide, a lipid second messenger. CONCLUSIONS. The results demonstrate that laser irradiation induces apoptosis in hRPE cells and suggest that the underlying signaling mechanism involves ceramide generation.",
author = "Adiel Barak and Tzipora Goldkorn and Morse, {Lawrence S}",
year = "2005",
doi = "10.1167/iovs.04-0920",
language = "English (US)",
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pages = "2587--2591",
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T1 - Laser induces apoptosis and ceramide production in human retinal pigment epithelial cells

AU - Barak, Adiel

AU - Goldkorn, Tzipora

AU - Morse, Lawrence S

PY - 2005

Y1 - 2005

N2 - PURPOSE. To investigate the cellular mechanisms involved in the cell death of human retinal pigment epithelial (hRPE) cells after their exposure to laser injury. METHODS. Cultured human hRPE cells were irradiated for different lengths of time and at different levels of energy using diode laser photocoagulation coupled with an intraocular laser probe. Apoptosis was determined by TUNEL staining and annexin-V labeling of phosphatidylserine exposure. Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatography. RESULTS. Laser irradiation caused areas of apoptosis in the hRPE cells. These areas were detected around the ablated and necrotic laser scar and developed several hours after the laser irradiation. Laser irradiation concomitantly induced an increase in the intracellular production of ceramide, a lipid second messenger. CONCLUSIONS. The results demonstrate that laser irradiation induces apoptosis in hRPE cells and suggest that the underlying signaling mechanism involves ceramide generation.

AB - PURPOSE. To investigate the cellular mechanisms involved in the cell death of human retinal pigment epithelial (hRPE) cells after their exposure to laser injury. METHODS. Cultured human hRPE cells were irradiated for different lengths of time and at different levels of energy using diode laser photocoagulation coupled with an intraocular laser probe. Apoptosis was determined by TUNEL staining and annexin-V labeling of phosphatidylserine exposure. Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatography. RESULTS. Laser irradiation caused areas of apoptosis in the hRPE cells. These areas were detected around the ablated and necrotic laser scar and developed several hours after the laser irradiation. Laser irradiation concomitantly induced an increase in the intracellular production of ceramide, a lipid second messenger. CONCLUSIONS. The results demonstrate that laser irradiation induces apoptosis in hRPE cells and suggest that the underlying signaling mechanism involves ceramide generation.

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