Laser Capture Microdissection of Feline Streptomyces spp Pyogranulomatous Dermatitis and Cellulitis

R. P. Traslavina, C. M. Reilly, R. Vasireddy, E. M. Samitz, C. T. Stepnik, Catherine A Outerbridge, Verena K Affolter, Barbara A Byrne, Linda J Lowenstine, Stephen D White, Brian G Murphy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Suspected Streptomyces spp infections were identified in 4 cats at UC Davis Veterinary Medical Teaching Hospital between 1982 and 2011. Three had ulcerated, dark red mycetomas involving the dermis, subcutis, and fascia with fistulous tracts and/or regional lymphadenopathy. One cat had pyogranulomatous mesenteric lymphadenitis. Granulomatous inflammation in all cats contained colonies of Gram-positive, non-acid-fast organisms. All 4 cats failed to respond to aggressive medical and surgical treatment and were euthanized. Laser capture microdissection (LCM) was used to selectively harvest DNA from the affected formalin-fixed, paraffin-embedded (FFPE) tissues. Cloned amplicons from LCM-derived tissue confirmed the presence of Streptomyces spp in the dermatitis cases. Amplicons from the remaining cat with peritoneal involvement aligned with the 16S ribosomal RNA gene for Actinomycetales. Usually considered a contaminant, Streptomyces spp can be associated with refractory pyogranulomatous dermatitis and cellulitis in cats with outdoor access. LCM is useful in the diagnosis of bacterial diseases where contamination may be an issue.

Original languageEnglish (US)
Pages (from-to)1172-1175
Number of pages4
JournalVeterinary Pathology
Volume52
Issue number6
DOIs
StatePublished - Nov 1 2015

Fingerprint

Laser Capture Microdissection
cellulitis
Cellulitis
Felidae
Streptomyces
Dermatitis
dermatitis
Cats
cats
Mesenteric Lymphadenitis
Actinomycetales
16S Ribosomal RNA
Mycetoma
lymphadenitis
fascia
lymphatic diseases
dermis
Fascia
Dermis
rRNA Genes

Keywords

  • Actinomycetales
  • cats
  • cellulitis
  • dermatitis
  • laser capture microdissection
  • mycetoma
  • Streptomyces

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Laser Capture Microdissection of Feline Streptomyces spp Pyogranulomatous Dermatitis and Cellulitis. / Traslavina, R. P.; Reilly, C. M.; Vasireddy, R.; Samitz, E. M.; Stepnik, C. T.; Outerbridge, Catherine A; Affolter, Verena K; Byrne, Barbara A; Lowenstine, Linda J; White, Stephen D; Murphy, Brian G.

In: Veterinary Pathology, Vol. 52, No. 6, 01.11.2015, p. 1172-1175.

Research output: Contribution to journalArticle

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