Laryngeal transplantation in minipigs

Early immunological outcomes

M. A. Birchall, S. M. Ayling, R. Harley, P. J. Murison, R. Burt, L. Mitchard, A. Jones, P. Macchiarini, C. R. Stokes, M. Bailey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Despite recent tissue-engineering advances, there is no effective way of replacing all the functions of the larynx in those requiring laryngectomy. A recent clinical transplant was a success. Using quantitative immunofluorescence targeted at immunologically relevant molecules, we have studied the early (48h and 1 week) immunological responses within larynxes transplantated between seven pairs of National Institutes of Health (NIH) minipigs fully homozygous at the major histocompatibility complex (MHC) locus. There were only small changes in expression of some molecules (relative to interindividual variation) and these were clearest in samples from the subglottic region, where the areas of co-expression of CD25 +CD45RC -CD8 - and of CD163 +CD172 +MHC-II - increased at 1 week after transplant. In one case, infiltration by recipient T cells was analysed by T cell receptor (TCR) Vβ spectratype analysis; this suggested that changes in the T cell repertoire occur in the donor subglottis mucosal tissues from day 0 to day 7, but that the donor and recipient mucosal Vβ repertoires remain distinct. The observed lack of strong immunological responses to the trauma of surgery and ischaemia provides encouraging evidence to support clinical trials of laryngeal transplantation, and a basis on which to interpret future studies involving mismatches.

Original languageEnglish (US)
Pages (from-to)556-564
Number of pages9
JournalClinical and Experimental Immunology
Volume167
Issue number3
DOIs
StatePublished - Mar 2012
Externally publishedYes

Fingerprint

Miniature Swine
Larynx
Major Histocompatibility Complex
Transplantation
T-Lymphocytes
Transplants
Laryngectomy
National Institutes of Health (U.S.)
Tissue Engineering
T-Cell Antigen Receptor
Fluorescent Antibody Technique
Mucous Membrane
Ischemia
Clinical Trials
Wounds and Injuries

Keywords

  • Macrophages/monocytes
  • T cells
  • Transplantation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Birchall, M. A., Ayling, S. M., Harley, R., Murison, P. J., Burt, R., Mitchard, L., ... Bailey, M. (2012). Laryngeal transplantation in minipigs: Early immunological outcomes. Clinical and Experimental Immunology, 167(3), 556-564. https://doi.org/10.1111/j.1365-2249.2011.04531.x

Laryngeal transplantation in minipigs : Early immunological outcomes. / Birchall, M. A.; Ayling, S. M.; Harley, R.; Murison, P. J.; Burt, R.; Mitchard, L.; Jones, A.; Macchiarini, P.; Stokes, C. R.; Bailey, M.

In: Clinical and Experimental Immunology, Vol. 167, No. 3, 03.2012, p. 556-564.

Research output: Contribution to journalArticle

Birchall, MA, Ayling, SM, Harley, R, Murison, PJ, Burt, R, Mitchard, L, Jones, A, Macchiarini, P, Stokes, CR & Bailey, M 2012, 'Laryngeal transplantation in minipigs: Early immunological outcomes', Clinical and Experimental Immunology, vol. 167, no. 3, pp. 556-564. https://doi.org/10.1111/j.1365-2249.2011.04531.x
Birchall MA, Ayling SM, Harley R, Murison PJ, Burt R, Mitchard L et al. Laryngeal transplantation in minipigs: Early immunological outcomes. Clinical and Experimental Immunology. 2012 Mar;167(3):556-564. https://doi.org/10.1111/j.1365-2249.2011.04531.x
Birchall, M. A. ; Ayling, S. M. ; Harley, R. ; Murison, P. J. ; Burt, R. ; Mitchard, L. ; Jones, A. ; Macchiarini, P. ; Stokes, C. R. ; Bailey, M. / Laryngeal transplantation in minipigs : Early immunological outcomes. In: Clinical and Experimental Immunology. 2012 ; Vol. 167, No. 3. pp. 556-564.
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