Laryngeal abductor muscle reinnervation in a pig model

Martin Birchall, Bernadine Idowu, Pamela Murison, Alan Jones, Rachel Burt, Sarah Ayling, Chris Stokes, Laysan Pope, Giorgio Terenghi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective-To develop a large animal model for studies of laryngeal abductor reinnervation. Material and Methods-Six minipigs underwent unilateral anastomosis of the phrenic nerve-abductor branch of the recurrent laryngeal nerve (RLN). Polyhydroxybutyrate (PHB) conduits were used for repair. At each of 30, 60 and 120 days, 2 animals underwent video laryngeal endoscopy (VLE) and were then killed. VLE was also performed in the 120-day pair at 60 days. Nerve-conduit-nerve-muscle samples were fixed for light and immunofluorescence (pan-neurofilaments, S-100) microscopy. Laryngeal muscles were harvested (myosin heavy chain analysis). Results-VLE showed recovery of abductor function in 1 animal at 60 days and in 1 at 120 days. Haematoxylin-eosin staining demonstrated a complex inflammatory response. Eosinophil recruitment was observed. Stepwise regeneration and reorganization of the distal nerve between 30 and 120 days was observed with pan-NF staining. The mean minimum diameter in the reinnervated posterior crico-arytenoids tended to increase for up to 120 days. Conclusions-Anastomosis of the phrenic nerve-abductor branch of the RLN with a PHB conduit in a pig can result in functional and histological recovery within 2-4 months and appears to at least sustain abductor muscle fibre morphology. Recovery occurs despite a complex inflammatory response, which may be an essential part of healing rather than inhibitory.

Original languageEnglish (US)
Pages (from-to)839-846
Number of pages8
JournalActa Oto-Laryngologica
Volume124
Issue number7
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Laryngeal Muscles
Endoscopy
Recurrent Laryngeal Nerve
Phrenic Nerve
Swine
Staining and Labeling
Miniature Swine
Muscles
Intermediate Filaments
Myosin Heavy Chains
Recovery of Function
Hematoxylin
Eosine Yellowish-(YS)
Eosinophils
Fluorescent Antibody Technique
Regeneration
Microscopy
Animal Models
Light

Keywords

  • Larynx
  • Phrenic nerve
  • Pig
  • Recurrent laryngeal nerve
  • Reinnervation

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Birchall, M., Idowu, B., Murison, P., Jones, A., Burt, R., Ayling, S., ... Terenghi, G. (2004). Laryngeal abductor muscle reinnervation in a pig model. Acta Oto-Laryngologica, 124(7), 839-846. https://doi.org/10.1080/00016480410022507

Laryngeal abductor muscle reinnervation in a pig model. / Birchall, Martin; Idowu, Bernadine; Murison, Pamela; Jones, Alan; Burt, Rachel; Ayling, Sarah; Stokes, Chris; Pope, Laysan; Terenghi, Giorgio.

In: Acta Oto-Laryngologica, Vol. 124, No. 7, 2004, p. 839-846.

Research output: Contribution to journalArticle

Birchall, M, Idowu, B, Murison, P, Jones, A, Burt, R, Ayling, S, Stokes, C, Pope, L & Terenghi, G 2004, 'Laryngeal abductor muscle reinnervation in a pig model', Acta Oto-Laryngologica, vol. 124, no. 7, pp. 839-846. https://doi.org/10.1080/00016480410022507
Birchall M, Idowu B, Murison P, Jones A, Burt R, Ayling S et al. Laryngeal abductor muscle reinnervation in a pig model. Acta Oto-Laryngologica. 2004;124(7):839-846. https://doi.org/10.1080/00016480410022507
Birchall, Martin ; Idowu, Bernadine ; Murison, Pamela ; Jones, Alan ; Burt, Rachel ; Ayling, Sarah ; Stokes, Chris ; Pope, Laysan ; Terenghi, Giorgio. / Laryngeal abductor muscle reinnervation in a pig model. In: Acta Oto-Laryngologica. 2004 ; Vol. 124, No. 7. pp. 839-846.
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AU - Pope, Laysan

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