Abstract
The increasing recognition of the roles of carbohydrates in fundamental biological processes and their potential as new therapeutics has accentuated the requirement for access to large quantities of varying carbohydrate structures. During the past two years, tremendous advances have been made in the large-scale enzymatic synthesis of oligosaccharides using isolated enzymes or engineered whole cells. Glycosidase mutants, namely glycosynthases, have been constructed to increase both the yields and selectivity of the glycoside bond formation. The use of glycosyltransferases in oligosaccharide synthesis has been further improved by fusing a glycosyltransferase with a sugar nucleotide-synthesizing enzyme and by various routes of in situ sugar nucleotide regeneration. Moreover, several sugar nucleotides and oligosaccharides were produced economically by a combination of recombinant microorganisms harboring sugar nucleotide biosynthesis genes and a glycosyltransferase gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 756-763 |
| Number of pages | 8 |
| Journal | Current Opinion in Drug Discovery and Development |
| Volume | 3 |
| Issue number | 6 |
| State | Published - 2000 |
| Externally published | Yes |
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Keywords
- Biopathway engineering
- Enzymatic synthesis
- Glycosidases
- Glycosyltransferases
- Oligosaccharides
- Whole-cell reaction
ASJC Scopus subject areas
- Molecular Medicine
- Drug Discovery
- Pharmacology
Cite this
Large-scale enzymatic synthesis of oligosaccharides. / Chen, Xi; Kowal, P.; Wang, P. G.
In: Current Opinion in Drug Discovery and Development, Vol. 3, No. 6, 2000, p. 756-763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large-scale enzymatic synthesis of oligosaccharides
AU - Chen, Xi
AU - Kowal, P.
AU - Wang, P. G.
PY - 2000
Y1 - 2000
N2 - The increasing recognition of the roles of carbohydrates in fundamental biological processes and their potential as new therapeutics has accentuated the requirement for access to large quantities of varying carbohydrate structures. During the past two years, tremendous advances have been made in the large-scale enzymatic synthesis of oligosaccharides using isolated enzymes or engineered whole cells. Glycosidase mutants, namely glycosynthases, have been constructed to increase both the yields and selectivity of the glycoside bond formation. The use of glycosyltransferases in oligosaccharide synthesis has been further improved by fusing a glycosyltransferase with a sugar nucleotide-synthesizing enzyme and by various routes of in situ sugar nucleotide regeneration. Moreover, several sugar nucleotides and oligosaccharides were produced economically by a combination of recombinant microorganisms harboring sugar nucleotide biosynthesis genes and a glycosyltransferase gene.
AB - The increasing recognition of the roles of carbohydrates in fundamental biological processes and their potential as new therapeutics has accentuated the requirement for access to large quantities of varying carbohydrate structures. During the past two years, tremendous advances have been made in the large-scale enzymatic synthesis of oligosaccharides using isolated enzymes or engineered whole cells. Glycosidase mutants, namely glycosynthases, have been constructed to increase both the yields and selectivity of the glycoside bond formation. The use of glycosyltransferases in oligosaccharide synthesis has been further improved by fusing a glycosyltransferase with a sugar nucleotide-synthesizing enzyme and by various routes of in situ sugar nucleotide regeneration. Moreover, several sugar nucleotides and oligosaccharides were produced economically by a combination of recombinant microorganisms harboring sugar nucleotide biosynthesis genes and a glycosyltransferase gene.
KW - Biopathway engineering
KW - Enzymatic synthesis
KW - Glycosidases
KW - Glycosyltransferases
KW - Oligosaccharides
KW - Whole-cell reaction
UR - http://www.scopus.com/inward/record.url?scp=0034528680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034528680&partnerID=8YFLogxK
M3 - Article
C2 - 19649904
AN - SCOPUS:0034528680
VL - 3
SP - 756
EP - 763
JO - Current Opinion in Drug Discovery and Development
JF - Current Opinion in Drug Discovery and Development
SN - 1367-6733
IS - 6
ER -