Large movement in the C terminus of CLC-0 chloride channel during slow gating

Ekaterina A. Bykova, Xiao Dong Zhang, Tsung Yu Chen, Jie Zheng

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

Chloride channels and transporters of the CLC gene family are expressed in virtually all cell types and are crucial in the regulation of membrane potential, chloride homeostasis and intravesicular pH. There are two gating processes that open CLC channels - fast and slow. The fast gating process in CLC channels has recently been linked to a small movement of a glutamate side chain. However, the molecular mechanism underlying the slow gating process is still elusive. Using spectroscopic microscopy, we observed a large backbone movement in the C terminus of the CLC-0 chloride channel that was functionally linked to slow gating. We further showed that the C-terminal movement had a time course similar to slow gating. In addition, a mutation known to lock the slow gate open prevented movement of the C terminus. When combined with recent structural information on the CLC C terminus, our findings provide a structural model for understanding the conformational changes linked to slow gating in CLC transport proteins.

Original languageEnglish (US)
Pages (from-to)1115-1119
Number of pages5
JournalNature Structural and Molecular Biology
Volume13
Issue number12
DOIs
StatePublished - Dec 11 2006

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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