Laminaria japonica Extract Enhances Intestinal Barrier Function by Altering Inflammatory Response and Tight Junction-Related Protein in Lipopolysaccharide-Stimulated Caco-2 Cells

Hyo Seon Yang, Fawaz Haj, Myoungsook Lee, Inhae Kang, Guiguo Zhang, Yunkyoung Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the normal physiological state, intestinal epithelial cells act as a defensive frontline of host mucosal immunity to tolerate constant exposure to external stimuli. In this study, we investigated the potential anti-inflammatory and gut permeability protective effects of Laminaria japonica (LJ) water extract (LJE) and three types of fermented Laminaria japonica water extracts (LJE-F1, LJE-F2, and LJE-F3) in lipopolysaccharide (LPS)-stimulated Caco-2, human intestinal epithelial cells. All four extracts significantly decreased the production of nitric oxide and interleukin-6 induced by LPS stimulus. In addition, LJE and the three types of LJE-Fs also inhibited LPS-induced loss of monolayer permeability, as assessed by changes in transepithelial electrical resistance. All four LJ extracts significantly prevented the inhibition of the protein levels of occludin, whereas LJE, LJE-F1, and LJE-F3 significantly attenuated the reduction in phosphorylation of adenosine monophosphate-activated protein kinase compared with the LPS-treated group in Caco-2 cells. In conclusion, LJE and its fermented water extracts appear to have potential gut health-promoting effects by reducing inflammation and partially regulating the tight junction-related proteins in human intestinal epithelial cells. Thus, additional studies are warranted to evaluate Laminaria japonica as a therapeutic agent for inflammatory bowel diseases.

Original languageEnglish (US)
JournalNutrients
Volume11
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Laminaria
Saccharina japonica
Tight Junction Proteins
tight junctions
Caco-2 Cells
lipopolysaccharides
Lipopolysaccharides
inflammation
Water
extracts
proteins
cells
water
Epithelial Cells
epithelial cells
Permeability
permeability
Occludin
digestive system
Mucosal Immunity

Keywords

  • anti-inflammatory
  • Caco-2 cells
  • fermentation
  • gut permeability
  • inflammatory bowel disease
  • Laminaria japonica

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Laminaria japonica Extract Enhances Intestinal Barrier Function by Altering Inflammatory Response and Tight Junction-Related Protein in Lipopolysaccharide-Stimulated Caco-2 Cells. / Yang, Hyo Seon; Haj, Fawaz; Lee, Myoungsook; Kang, Inhae; Zhang, Guiguo; Lee, Yunkyoung.

In: Nutrients, Vol. 11, No. 5, 01.05.2019.

Research output: Contribution to journalArticle

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