Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis

Christopher T. Simons, Yves Boucher, Mirela Iodi Carstens, Earl Carstens

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.

Original languageEnglish (US)
Pages (from-to)253-259
Number of pages7
JournalChemical Senses
Volume28
Issue number3
DOIs
StatePublished - Mar 2003

Fingerprint

quinine
Quinine
Pentanoic Acids
rats
tongue
Tongue
acids
neurons
Neurons
electrophysiology
Electrophysiology
brain stem
Brain Stem
immunohistochemistry
Hot Temperature
Immunohistochemistry
heat
Water
water

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Neuroscience(all)
  • Physiology
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Simons, C. T., Boucher, Y., Carstens, M. I., & Carstens, E. (2003). Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis. Chemical Senses, 28(3), 253-259. https://doi.org/10.1093/chemse/28.3.253

Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis. / Simons, Christopher T.; Boucher, Yves; Carstens, Mirela Iodi; Carstens, Earl.

In: Chemical Senses, Vol. 28, No. 3, 03.2003, p. 253-259.

Research output: Contribution to journalArticle

Simons, CT, Boucher, Y, Carstens, MI & Carstens, E 2003, 'Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis', Chemical Senses, vol. 28, no. 3, pp. 253-259. https://doi.org/10.1093/chemse/28.3.253
Simons, Christopher T. ; Boucher, Yves ; Carstens, Mirela Iodi ; Carstens, Earl. / Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis. In: Chemical Senses. 2003 ; Vol. 28, No. 3. pp. 253-259.
@article{fd6f3424bac1466d9889f1170b5fb190,
title = "Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis",
abstract = "Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.",
author = "Simons, {Christopher T.} and Yves Boucher and Carstens, {Mirela Iodi} and Earl Carstens",
year = "2003",
month = "3",
doi = "10.1093/chemse/28.3.253",
language = "English (US)",
volume = "28",
pages = "253--259",
journal = "Chemical Senses",
issn = "0379-864X",
publisher = "Oxford University Press",
number = "3",

}

TY - JOUR

T1 - Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis

AU - Simons, Christopher T.

AU - Boucher, Yves

AU - Carstens, Mirela Iodi

AU - Carstens, Earl

PY - 2003/3

Y1 - 2003/3

N2 - Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.

AB - Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.

UR - http://www.scopus.com/inward/record.url?scp=0642279429&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0642279429&partnerID=8YFLogxK

U2 - 10.1093/chemse/28.3.253

DO - 10.1093/chemse/28.3.253

M3 - Article

C2 - 12714448

AN - SCOPUS:0642279429

VL - 28

SP - 253

EP - 259

JO - Chemical Senses

JF - Chemical Senses

SN - 0379-864X

IS - 3

ER -