Abstract
Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 253-259 |
| Number of pages | 7 |
| Journal | Chemical Senses |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 2003 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Food Science
- Neuroscience(all)
- Physiology
- Physiology (medical)
- Behavioral Neuroscience
Cite this
Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis. / Simons, Christopher T.; Boucher, Yves; Carstens, Mirela Iodi; Carstens, Earl.
In: Chemical Senses, Vol. 28, No. 3, 03.2003, p. 253-259.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of quinine-evoked activity in rat trigeminal subnucleus caudalis
AU - Simons, Christopher T.
AU - Boucher, Yves
AU - Carstens, Mirela Iodi
AU - Carstens, Earl
PY - 2003/3
Y1 - 2003/3
N2 - Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.
AB - Conflicting reports exist regarding the ability of quinine to activate neurons in the trigeminal system. We used the complementary approaches of single-unit electrophysiology and c-fos immunohistochemistry to investigate whether quinine (100 mM) activates chemonociceptive cells in the brainstem trigeminal subnucleus caudalis (Vc). In electrophysiological experiments, 38 units responded to noxious mechanical, thermal and chemical (200 mM pentanoic acid) stimuli applied to the tongue with an increase in firing rate; none responded to lingual quinine whether the quinine was presented before or after application of pentanoic acid. In the c-fos immunohistochemical experiment, both quinine and water elicited equivalent levels of fos-like immunoreactivity (FLI) in dorsomedial Vc that were significantly lower than the level of FLI evoked by pentanoic acid. These data collectively indicate that quinine does not elicit activity in chemonociceptive Vc neurons.
UR - http://www.scopus.com/inward/record.url?scp=0642279429&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0642279429&partnerID=8YFLogxK
U2 - 10.1093/chemse/28.3.253
DO - 10.1093/chemse/28.3.253
M3 - Article
C2 - 12714448
AN - SCOPUS:0642279429
VL - 28
SP - 253
EP - 259
JO - Chemical Senses
JF - Chemical Senses
SN - 0379-864X
IS - 3
ER -