Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy

David M. Sherry, Anne R. Murray, Yogita Kanan, Kelsey L. Arbogast, Robert A. Hamilton, Steven J. Fliesler, Marie E Burns, Kevin L. Moore, Muayyad R. Al-Ubaidi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To investigate the role(s) of protein-tyrosine sulfation in the retina, we examined retinal function and structure in mice lacking tyrosylprotein sulfotransferases (TPST) 1 and 2. Tpst double knockout (DKO; Tpst1-/-//Tpst2-/-) retinas had drastically reduced electroretinographic responses, although their photoreceptors exhibited normal responses in single cell recordings. These retinas appeared normal histologically; however, the rod photoreceptors had ultrastructurally abnormal outer segments, with membrane evulsions into the extracellular space, irregular disc membrane spacing and expanded intradiscal space. Photoreceptor synaptic terminals were disorganized in Tpst DKO retinas, but established ultrastructurally normal synapses, as did bipolar and amacrine cells; however, the morphology and organization of neuronal processes in the inner retina were abnormal. These results indicate that protein-tyrosine sulfation is essential for proper outer segment morphogenesis and synaptic function, but is not critical for overall retinal structure or synapse formation, and may serve broader functions in neuronal development and maintenance.

Original languageEnglish (US)
Pages (from-to)1461-1472
Number of pages12
JournalEuropean Journal of Neuroscience
Volume32
Issue number9
DOIs
StatePublished - Nov 2010

Fingerprint

Retinal Photoreceptor Cell Outer Segment
Morphogenesis
Tyrosine
Retina
Anatomy
Proteins
Synapses
Retinal Rod Photoreceptor Cells
Amacrine Cells
Membranes
Presynaptic Terminals
Extracellular Space
Maintenance

Keywords

  • Extracellular matrix
  • Mouse
  • Retina
  • Retinal degeneration
  • Tyrosine O-sulfation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sherry, D. M., Murray, A. R., Kanan, Y., Arbogast, K. L., Hamilton, R. A., Fliesler, S. J., ... Al-Ubaidi, M. R. (2010). Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy. European Journal of Neuroscience, 32(9), 1461-1472. https://doi.org/10.1111/j.1460-9568.2010.07431.x

Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy. / Sherry, David M.; Murray, Anne R.; Kanan, Yogita; Arbogast, Kelsey L.; Hamilton, Robert A.; Fliesler, Steven J.; Burns, Marie E; Moore, Kevin L.; Al-Ubaidi, Muayyad R.

In: European Journal of Neuroscience, Vol. 32, No. 9, 11.2010, p. 1461-1472.

Research output: Contribution to journalArticle

Sherry, DM, Murray, AR, Kanan, Y, Arbogast, KL, Hamilton, RA, Fliesler, SJ, Burns, ME, Moore, KL & Al-Ubaidi, MR 2010, 'Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy', European Journal of Neuroscience, vol. 32, no. 9, pp. 1461-1472. https://doi.org/10.1111/j.1460-9568.2010.07431.x
Sherry, David M. ; Murray, Anne R. ; Kanan, Yogita ; Arbogast, Kelsey L. ; Hamilton, Robert A. ; Fliesler, Steven J. ; Burns, Marie E ; Moore, Kevin L. ; Al-Ubaidi, Muayyad R. / Lack of protein-tyrosine sulfation disrupts photoreceptor outer segment morphogenesis, retinal function and retinal anatomy. In: European Journal of Neuroscience. 2010 ; Vol. 32, No. 9. pp. 1461-1472.
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