Lack of protection against avian cholera by vaccination with recombinant P6-like protein from pasteurella multocida

Rickie W. Kasten, Patricia S. Wakenell, Shabbir Ahmad, Tilahun Yilma, Dwight C. Hirsh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The gene encoding the P6-like protein of Pasteurella multocida was cloned in the baculovirus expression system. Baculovirus-expressed recombinant protein was used to parenterally immunize 6-wk-old Nicholas broad-breasted white turkeys. Turkeys developed significant antibody titers to the recombinant protein as measured by enzyme-linked immunosorbent assay. Two weeks after the last immunizing injection, vaccinated turkeys were placed in contact with turkeys infected with P. multocida strain P1059, as were nonvaccinated control birds. No differences occurred in percent mortality between the two groups. We conclude that parenterally administered recombinant P6-like protein does not protect turkeys from avian cholera.

Original languageEnglish (US)
Pages (from-to)972-976
Number of pages5
JournalAvian Diseases
Volume41
Issue number4
StatePublished - Oct 1997

Fingerprint

cholera
Cholera
Pasteurella multocida
Vaccination
vaccination
Baculoviridae
Recombinant Proteins
recombinant proteins
proteins
bird control
Birds
Enzyme-Linked Immunosorbent Assay
Pasteurella multocida P6-like protein
enzyme-linked immunosorbent assay
injection
Injections
antibodies
Mortality
Antibodies
Genes

Keywords

  • Avian cholera
  • Pasteurella multocida
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Kasten, R. W., Wakenell, P. S., Ahmad, S., Yilma, T., & Hirsh, D. C. (1997). Lack of protection against avian cholera by vaccination with recombinant P6-like protein from pasteurella multocida. Avian Diseases, 41(4), 972-976.

Lack of protection against avian cholera by vaccination with recombinant P6-like protein from pasteurella multocida. / Kasten, Rickie W.; Wakenell, Patricia S.; Ahmad, Shabbir; Yilma, Tilahun; Hirsh, Dwight C.

In: Avian Diseases, Vol. 41, No. 4, 10.1997, p. 972-976.

Research output: Contribution to journalArticle

Kasten, RW, Wakenell, PS, Ahmad, S, Yilma, T & Hirsh, DC 1997, 'Lack of protection against avian cholera by vaccination with recombinant P6-like protein from pasteurella multocida', Avian Diseases, vol. 41, no. 4, pp. 972-976.
Kasten, Rickie W. ; Wakenell, Patricia S. ; Ahmad, Shabbir ; Yilma, Tilahun ; Hirsh, Dwight C. / Lack of protection against avian cholera by vaccination with recombinant P6-like protein from pasteurella multocida. In: Avian Diseases. 1997 ; Vol. 41, No. 4. pp. 972-976.
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