Lack of interferon beta-induced radiosensitization in four out of five human glioblastoma cell lines

Heinz Schmidberger, Margret Rave-Fränk, Jörg Lehmann, Elisabeth Weiss, Lara Gerl, Nadine Dettmer, Sabine Glomme, C. F. Hess

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Purpose: To investigate the potential of interferon β to enhance the cytotoxic activity of ionizing irradiation against glioma cells, and to elucidate the possible mechanisms responsible for conflicting clinical results. Methods and Materials: Five glioblastoma cell lines (U87MG, U118MG, U373MG, MO59K, MO59J) with different radiosensitivity and genetic background were used. Experiments were performed in exponentially growing cultures, and cell survival was measured by a colony-forming assay. Cells were incubated with natural interferon β (n-IFN-β; 30-3000 IU/mL) for 24 h followed by single dose irradiation with 1 to 6 Gy of γ-rays. Results: Significant differences in n-IFN-β sensitivity were found. The cell lines also differed in their radiation sensitivity, and there was no correlation between the n-IFN-β and the radiation sensitivity. In three of five cell lines, the interaction of n-IFN-β and irradiation was infra-additive; in one cell line, it was additive. For MO59J cells only, which are NHEJ-deficient, supra-additivity was observed. Conclusion: Our results confirm the remarkable heterogeneity that is characteristic of malignant glioma. The combined effect of n-IFN-β and radiation was mostly infra-additive or additive; synergistic interaction might occur in tumor cells that already have acquired repair deficiencies because of their genetic instability, as shown for the MO59J cell line.

Original languageEnglish (US)
Pages (from-to)1348-1357
Number of pages10
JournalInternational Journal of Radiation Oncology Biology Physics
Volume55
Issue number5
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

interferon
Interferon-beta
Glioblastoma
cultured cells
Radiation Tolerance
Cell Line
Glioma
Interferons
irradiation
radiation
cells
radiation tolerance
Cell Communication
Cell Survival
rays
tumors
interactions
Radiation
dosage
sensitivity

Keywords

  • Glioblastoma
  • In vitro
  • Interferon beta
  • Radiosensitization

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Lack of interferon beta-induced radiosensitization in four out of five human glioblastoma cell lines. / Schmidberger, Heinz; Rave-Fränk, Margret; Lehmann, Jörg; Weiss, Elisabeth; Gerl, Lara; Dettmer, Nadine; Glomme, Sabine; Hess, C. F.

In: International Journal of Radiation Oncology Biology Physics, Vol. 55, No. 5, 01.04.2003, p. 1348-1357.

Research output: Contribution to journalArticle

Schmidberger, H, Rave-Fränk, M, Lehmann, J, Weiss, E, Gerl, L, Dettmer, N, Glomme, S & Hess, CF 2003, 'Lack of interferon beta-induced radiosensitization in four out of five human glioblastoma cell lines', International Journal of Radiation Oncology Biology Physics, vol. 55, no. 5, pp. 1348-1357. https://doi.org/10.1016/S0360-3016(02)04575-3
Schmidberger, Heinz ; Rave-Fränk, Margret ; Lehmann, Jörg ; Weiss, Elisabeth ; Gerl, Lara ; Dettmer, Nadine ; Glomme, Sabine ; Hess, C. F. / Lack of interferon beta-induced radiosensitization in four out of five human glioblastoma cell lines. In: International Journal of Radiation Oncology Biology Physics. 2003 ; Vol. 55, No. 5. pp. 1348-1357.
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