Abstract
Galectin-3 is a β-galactoside-binding lectin implicated in the fine-tuning of innate immunity. Rhodococcus equi, a facultative intracellular bacterium of macrophages, causes severe granulomatous bronchopneumonia in young horses and immunocompromised humans. The aim of this study is to investigate the role of galectin-3 in the innate resistance mechanism against R. equi infection. The bacterial challenge of galectin-3-deficient mice (gal3-/-) and their wild-type counterpart (gal3+/+) revealed that the LD50 for the gal3-/- mice was about seven times higher than that for the gal3+/+ mice. When challenged with a sublethal dose, gal3-/- mice showed lower bacteria counts and higher production of IL-12 and IFN-γ production, besides exhibiting a delayed although increased inflammatory reaction. Gal3-/- macrophages exhibited a decreased frequency of bacterial replication and survival, and higher transcript levels of IL-1β, IL-6, IL-10, TLR2 and MyD88. R. equi-infected gal3+/+ macrophages showed decreased expression of TLR2, whereas R. equi-infected gal3-/- macrophages showed enhanced expression of this receptor. Furthermore, galectin-3 deficiency in macrophages may be responsible for the higher IL-1β serum levels detected in infected gal3-/- mice. Therefore galectin-3 may exert a regulatory role in innate immunity by diminishing IL-1β production and thus affecting resistance to R. equi infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2762-2775 |
| Number of pages | 14 |
| Journal | European Journal of Immunology |
| Volume | 38 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2008 |
Fingerprint
Keywords
- Bacterial infections
- Galectin-3
- IL-1β
- Innate immunity
- Toll-like receptor
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Lack of galectin-3 alters the balance of innate immune cytokines and confers resistance to Rhodococcus equi infection. / Ferraz, Luciana C.; Bernardes, Emerson S.; Oliveira, Aline F.; Ruas, Luciana P.; Fermino, Marise L.; Soares, Sandro G.; Loyola, Adriano M.; Oliver, Constance; Jamur, Maria C.; Hsu, Daniel K.; Liu, Fu-Tong; Chammas, Roger; Roque-Barreira, Maria Cristina.
In: European Journal of Immunology, Vol. 38, No. 10, 2008, p. 2762-2775.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of galectin-3 alters the balance of innate immune cytokines and confers resistance to Rhodococcus equi infection
AU - Ferraz, Luciana C.
AU - Bernardes, Emerson S.
AU - Oliveira, Aline F.
AU - Ruas, Luciana P.
AU - Fermino, Marise L.
AU - Soares, Sandro G.
AU - Loyola, Adriano M.
AU - Oliver, Constance
AU - Jamur, Maria C.
AU - Hsu, Daniel K.
AU - Liu, Fu-Tong
AU - Chammas, Roger
AU - Roque-Barreira, Maria Cristina
PY - 2008
Y1 - 2008
N2 - Galectin-3 is a β-galactoside-binding lectin implicated in the fine-tuning of innate immunity. Rhodococcus equi, a facultative intracellular bacterium of macrophages, causes severe granulomatous bronchopneumonia in young horses and immunocompromised humans. The aim of this study is to investigate the role of galectin-3 in the innate resistance mechanism against R. equi infection. The bacterial challenge of galectin-3-deficient mice (gal3-/-) and their wild-type counterpart (gal3+/+) revealed that the LD50 for the gal3-/- mice was about seven times higher than that for the gal3+/+ mice. When challenged with a sublethal dose, gal3-/- mice showed lower bacteria counts and higher production of IL-12 and IFN-γ production, besides exhibiting a delayed although increased inflammatory reaction. Gal3-/- macrophages exhibited a decreased frequency of bacterial replication and survival, and higher transcript levels of IL-1β, IL-6, IL-10, TLR2 and MyD88. R. equi-infected gal3+/+ macrophages showed decreased expression of TLR2, whereas R. equi-infected gal3-/- macrophages showed enhanced expression of this receptor. Furthermore, galectin-3 deficiency in macrophages may be responsible for the higher IL-1β serum levels detected in infected gal3-/- mice. Therefore galectin-3 may exert a regulatory role in innate immunity by diminishing IL-1β production and thus affecting resistance to R. equi infection.
AB - Galectin-3 is a β-galactoside-binding lectin implicated in the fine-tuning of innate immunity. Rhodococcus equi, a facultative intracellular bacterium of macrophages, causes severe granulomatous bronchopneumonia in young horses and immunocompromised humans. The aim of this study is to investigate the role of galectin-3 in the innate resistance mechanism against R. equi infection. The bacterial challenge of galectin-3-deficient mice (gal3-/-) and their wild-type counterpart (gal3+/+) revealed that the LD50 for the gal3-/- mice was about seven times higher than that for the gal3+/+ mice. When challenged with a sublethal dose, gal3-/- mice showed lower bacteria counts and higher production of IL-12 and IFN-γ production, besides exhibiting a delayed although increased inflammatory reaction. Gal3-/- macrophages exhibited a decreased frequency of bacterial replication and survival, and higher transcript levels of IL-1β, IL-6, IL-10, TLR2 and MyD88. R. equi-infected gal3+/+ macrophages showed decreased expression of TLR2, whereas R. equi-infected gal3-/- macrophages showed enhanced expression of this receptor. Furthermore, galectin-3 deficiency in macrophages may be responsible for the higher IL-1β serum levels detected in infected gal3-/- mice. Therefore galectin-3 may exert a regulatory role in innate immunity by diminishing IL-1β production and thus affecting resistance to R. equi infection.
KW - Bacterial infections
KW - Galectin-3
KW - IL-1β
KW - Innate immunity
KW - Toll-like receptor
UR - http://www.scopus.com/inward/record.url?scp=58149240945&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149240945&partnerID=8YFLogxK
U2 - 10.1002/eji.200737986
DO - 10.1002/eji.200737986
M3 - Article
C2 - 18825751
AN - SCOPUS:58149240945
VL - 38
SP - 2762
EP - 2775
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 10
ER -