Abstract
Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 623-625 |
| Number of pages | 3 |
| Journal | Journal of Veterinary Internal Medicine |
| Volume | 23 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 2009 |
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Keywords
- Dermatology
- Horse
- Immunology
- Infectious disease
- PCR
ASJC Scopus subject areas
- veterinary(all)
Cite this
Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis. / White, Stephen D; Foley, Janet E; Spiegel, I. B.; Ihrke, P. J.
In: Journal of Veterinary Internal Medicine, Vol. 23, No. 3, 05.2009, p. 623-625.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis
AU - White, Stephen D
AU - Foley, Janet E
AU - Spiegel, I. B.
AU - Ihrke, P. J.
PY - 2009/5
Y1 - 2009/5
N2 - Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.
AB - Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.
KW - Dermatology
KW - Horse
KW - Immunology
KW - Infectious disease
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=67449102341&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67449102341&partnerID=8YFLogxK
U2 - 10.1111/j.1939-1676.2009.0291.x
DO - 10.1111/j.1939-1676.2009.0291.x
M3 - Article
C2 - 19645844
AN - SCOPUS:67449102341
VL - 23
SP - 623
EP - 625
JO - Journal of Veterinary Internal Medicine
JF - Journal of Veterinary Internal Medicine
SN - 0891-6640
IS - 3
ER -