Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis

Stephen D White, Janet E Foley, I. B. Spiegel, P. J. Ihrke

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.

Original languageEnglish (US)
Pages (from-to)623-625
Number of pages3
JournalJournal of Veterinary Internal Medicine
Volume23
Issue number3
DOIs
StatePublished - May 2009

Fingerprint

Rhadinovirus
Equid herpesvirus 2
Equid Herpesvirus 1
Equid herpesvirus 1
Sarcoidosis
Paraffin
alkanes
Horses
horses
Herpesviridae
polymerase chain reaction
DNA
Polymerase Chain Reaction
Viral DNA
skin diseases
Skin Diseases
retrospective studies
skin (animal)
Respiratory Tract Infections
etiology

Keywords

  • Dermatology
  • Horse
  • Immunology
  • Infectious disease
  • PCR

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis. / White, Stephen D; Foley, Janet E; Spiegel, I. B.; Ihrke, P. J.

In: Journal of Veterinary Internal Medicine, Vol. 23, No. 3, 05.2009, p. 623-625.

Research output: Contribution to journalArticle

@article{f26925505c854180a7e159c27cdcd6db,
title = "Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis",
abstract = "Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.",
keywords = "Dermatology, Horse, Immunology, Infectious disease, PCR",
author = "White, {Stephen D} and Foley, {Janet E} and Spiegel, {I. B.} and Ihrke, {P. J.}",
year = "2009",
month = "5",
doi = "10.1111/j.1939-1676.2009.0291.x",
language = "English (US)",
volume = "23",
pages = "623--625",
journal = "Journal of Veterinary Internal Medicine",
issn = "0891-6640",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Lack of detectable equine herpesviruses 1 and 2 in paraffin-embedded specimens of equine sarcoidosis

AU - White, Stephen D

AU - Foley, Janet E

AU - Spiegel, I. B.

AU - Ihrke, P. J.

PY - 2009/5

Y1 - 2009/5

N2 - Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.

AB - Background: Equine sarcoidosis is a rare, multisystemic, noncaseating, granulomatous and lymphoplasmacytic disease of unknown etiology. A recent report described a horse with granulomatous skin disease displaying histologic, electron microscopic, and polymerase chain reaction (PCR) findings consistent with equine herpesvirus 2 (EHV-2). Objective: To investigate the presence of EHV-2 and equine herpesvirus 1 (EHV-1) in 8 horses with sarcoidosis. Animals: Eight horses with sarcoidosis, reported previously. Methods: Retrospective study. PCR assays of the tissues were performed to detect DNA associated with EHV-1 and EHV-2. For both herpesviruses the target was their respective glycoprotein B gene. Positive controls consisted of DNA from viral cultures of culturettes from naturally occurring respiratory infections of EHV-1 and EHV-2. Results: The PCR analyses for both equine herpesviruses' DNA were negative in all 8 horses. Conclusion: The failure to detect DNA from EHV-1 and EHV-2 in paraffin-embedded skin of these 8 horses does not discount EHV-1 or EHV-2 as causing some cases of ES, but lends support to the presumably multifactorial etiologic nature of the disease.

KW - Dermatology

KW - Horse

KW - Immunology

KW - Infectious disease

KW - PCR

UR - http://www.scopus.com/inward/record.url?scp=67449102341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67449102341&partnerID=8YFLogxK

U2 - 10.1111/j.1939-1676.2009.0291.x

DO - 10.1111/j.1939-1676.2009.0291.x

M3 - Article

C2 - 19645844

AN - SCOPUS:67449102341

VL - 23

SP - 623

EP - 625

JO - Journal of Veterinary Internal Medicine

JF - Journal of Veterinary Internal Medicine

SN - 0891-6640

IS - 3

ER -