Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles

Ann Davidson, Josie L. Traub-Dargatz, Roberta Magnuson, Ashley E Hill, Vivienne Irwin, Richard Newton, Andrew Waller, Kenneth Smith, Robert J. Callan, Mary Meehan, Peter Owen, Mo Salman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previously published studies have neither used nor reported the results of an indirect enzyme-linked immunosorbent assay (iELISA) to measure serologic responses in natural outbreaks of strangles. The concept of using serologic responses to identify persistent carriers of Streptococcus equi has been proposed but not scientifically evaluated. The specific aims of the current study were to determine the duration and level of truncated fibrinogen-binding protein-specific (SeM allele 1) antibody production in ponies involved in a natural outbreak of strangles and to determine if test results from this serologic iELISA could predict persistent carrier status. Serologic samples were obtained before and after an outbreak of naturally occurring strangles infection. Persistent carriers of S. equi were identified via culture and polymerase chain reaction (PCR) testing of lavage fluid collected from the guttural pouches and nasopharynx or swabs of the nasopharynx after recovery from acute disease and at postmortem examination. Logistic regression analysis was used to determine if an association existed between serologic response and persistent carrier state. The ELISA reported in the current study definitively confirmed a recent exposure to S. equi. However, the measured serologic response did not predict carrier status in this strangles outbreak. Therefore, a guttural-pouch endoscopy with subsequent culture or PCR testing to detect S. equi remains the most accurate method available for the identification of persistent carriers.

Original languageEnglish (US)
Pages (from-to)457-462
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume20
Issue number4
StatePublished - Jul 1 2008
Externally publishedYes

Fingerprint

Streptococcus equi
strangles
fibrinogen
carrier state
Disease Outbreaks
binding proteins
guttural pouch
nasopharynx
antibodies
Nasopharynx
Antibodies
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Pharynx
polymerase chain reaction
Carrier State
acute course
Polymerase Chain Reaction
endoscopy
Therapeutic Irrigation

Keywords

  • Enzyme-linked immunosorbent assay
  • Persistent carrier
  • Serologic response
  • Strangles

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles. / Davidson, Ann; Traub-Dargatz, Josie L.; Magnuson, Roberta; Hill, Ashley E; Irwin, Vivienne; Newton, Richard; Waller, Andrew; Smith, Kenneth; Callan, Robert J.; Meehan, Mary; Owen, Peter; Salman, Mo.

In: Journal of Veterinary Diagnostic Investigation, Vol. 20, No. 4, 01.07.2008, p. 457-462.

Research output: Contribution to journalArticle

Davidson, A, Traub-Dargatz, JL, Magnuson, R, Hill, AE, Irwin, V, Newton, R, Waller, A, Smith, K, Callan, RJ, Meehan, M, Owen, P & Salman, M 2008, 'Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles', Journal of Veterinary Diagnostic Investigation, vol. 20, no. 4, pp. 457-462.
Davidson, Ann ; Traub-Dargatz, Josie L. ; Magnuson, Roberta ; Hill, Ashley E ; Irwin, Vivienne ; Newton, Richard ; Waller, Andrew ; Smith, Kenneth ; Callan, Robert J. ; Meehan, Mary ; Owen, Peter ; Salman, Mo. / Lack of correlation between antibody titers to fibrinogen-binding protein of Streptococcus equi and persistent carriers of strangles. In: Journal of Veterinary Diagnostic Investigation. 2008 ; Vol. 20, No. 4. pp. 457-462.
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