Laboratory maintenance of Brucella abortus.

Yao Hui Sun, Andreas B. den Hartigh, Renee M Tsolis, Thomas A. Ficht

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

This unit provides protocols for growth of Brucella abortus on solid or in liquid media and for long-term storage of laboratory stocks. Two issues affecting the culture and storage of isolates of this slow-growing bacterium are emphasized: contamination of cultures and outgrowth of attenuated variants lacking a complete lipopolysaccharide. Laboratories planning to work with B. abortus should be aware that Biosafety Level 3 facilities are required. Furthermore, this organism is classified in the U.S. as a Select Agent, which restricts its use to laboratories registered with the U.S. government's Select Agent programs.

Original languageEnglish (US)
JournalCurrent Protocols in Microbiology
VolumeChapter 3
StatePublished - Oct 2005

ASJC Scopus subject areas

  • Microbiology
  • Parasitology
  • Virology

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