Labeling ribonuclease S with a 3 nm Au nanoparticle by two-step assembly

Marie Eve Aubin, Diana G. Morales, Kimberly Hamad-Schifferli

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We label ribonuclease S with a 3 nm Au nanoparticle (NP) by utilizing its two-piece structure. One portion, S-peptide, is mutated with a unique NP attachment site. NP-peptide self-assembles with the other portion, S-protein, to form an active enzyme. NP mobility decreases with peptide labeling and S-protein association. Surface plasmon shifts support conjugation. Higher S-peptide coverages on the NP surface reduce nonspecific adsorption, while sterically hindering assembly of RNaseS. Thiols displace nonspecific adsorption, maximizing site-specific labeling.

Original languageEnglish (US)
Pages (from-to)519-522
Number of pages4
JournalNano Letters
Volume5
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

ASJC Scopus subject areas

  • Materials Science(all)
  • Electronic, Optical and Magnetic Materials
  • Chemistry (miscellaneous)

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    Aubin, M. E., Morales, D. G., & Hamad-Schifferli, K. (2005). Labeling ribonuclease S with a 3 nm Au nanoparticle by two-step assembly. Nano Letters, 5(3), 519-522. https://doi.org/10.1021/nl0479031