Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cellderived cardiomyocytes

Rupsa Datta, Christopher Heylman, Steven George, Enrico Gratton

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In this work we demonstrate a label-free optical imaging technique to assess metabolic status and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes by two-photon fluorescence lifetime imaging of endogenous fluorophores. Our results show the sensitivity of this method to detect shifts in metabolism and oxidative stress in the cardiomyocytes upon pathological stimuli of hypoxia and cardiotoxic drugs. This non-invasive imaging technique could prove beneficial for drug development and screening, especially for in vitro cardiac models created from stem cell-derived cardiomyocytes and to study the pathogenesis of cardiac diseases and therapy.

Original languageEnglish (US)
Pages (from-to)1690-1701
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number5
DOIs
StatePublished - May 1 2016
Externally publishedYes

Fingerprint

stem cells
metabolism
stems
Cardiac Myocytes
imaging techniques
drugs
Oxidative Stress
Optical Imaging
pathogenesis
hypoxia
stimuli
Induced Pluripotent Stem Cells
therapy
Preclinical Drug Evaluations
screening
Photons
life (durability)
fluorescence
sensitivity
Heart Diseases

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cellderived cardiomyocytes. / Datta, Rupsa; Heylman, Christopher; George, Steven; Gratton, Enrico.

In: Biomedical Optics Express, Vol. 7, No. 5, 01.05.2016, p. 1690-1701.

Research output: Contribution to journalArticle

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