Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cellderived cardiomyocytes

Rupsa Datta, Christopher Heylman, Steven George, Enrico Gratton

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

In this work we demonstrate a label-free optical imaging technique to assess metabolic status and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes by two-photon fluorescence lifetime imaging of endogenous fluorophores. Our results show the sensitivity of this method to detect shifts in metabolism and oxidative stress in the cardiomyocytes upon pathological stimuli of hypoxia and cardiotoxic drugs. This non-invasive imaging technique could prove beneficial for drug development and screening, especially for in vitro cardiac models created from stem cell-derived cardiomyocytes and to study the pathogenesis of cardiac diseases and therapy.

Original languageEnglish (US)
Pages (from-to)1690-1701
Number of pages12
JournalBiomedical Optics Express
Volume7
Issue number5
DOIs
StatePublished - May 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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