Label-free biochemical characterization of stem cells using vibrational spectroscopy

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Raman and infrared (IR) spectroscopy are two complementary vibrational spectroscopic techniques that have experienced a tremendous growth in their use in biological and biomedical research. This is, in large part, due to their unique capability of providing label-free intrinsic chemical information of living biological samples at tissue, cellular, or sub-cellular resolutions. This article reviews recent developments in applying these techniques for the characterization of stem cells. A discussion of the potential for these methods to address some of the major challenges in stem cell research is presented, as well as the technological and scientific advancements that are needed to progress the knowledge in the field.

Original languageEnglish (US)
Pages (from-to)656-668
Number of pages13
JournalJournal of Biophotonics
Volume2
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Stem Cell Research
Vibrational spectroscopy
Raman Spectrum Analysis
stem cells
Stem cells
Biomedical Research
Labels
Spectrum Analysis
Stem Cells
Growth
spectroscopy
Raman spectroscopy
Infrared spectroscopy
infrared spectroscopy
Tissue

Keywords

  • Adult stem cells
  • Embryonic stem cells
  • Infrared spectroscopy
  • Mesenchymal stem cells
  • Raman spectroscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Label-free biochemical characterization of stem cells using vibrational spectroscopy. / Chan, James W; Lieu, Deborah.

In: Journal of Biophotonics, Vol. 2, No. 11, 11.2009, p. 656-668.

Research output: Contribution to journalArticle

@article{dd8c6e4170ed47f8ba47b0807cff14c2,
title = "Label-free biochemical characterization of stem cells using vibrational spectroscopy",
abstract = "Raman and infrared (IR) spectroscopy are two complementary vibrational spectroscopic techniques that have experienced a tremendous growth in their use in biological and biomedical research. This is, in large part, due to their unique capability of providing label-free intrinsic chemical information of living biological samples at tissue, cellular, or sub-cellular resolutions. This article reviews recent developments in applying these techniques for the characterization of stem cells. A discussion of the potential for these methods to address some of the major challenges in stem cell research is presented, as well as the technological and scientific advancements that are needed to progress the knowledge in the field.",
keywords = "Adult stem cells, Embryonic stem cells, Infrared spectroscopy, Mesenchymal stem cells, Raman spectroscopy",
author = "Chan, {James W} and Deborah Lieu",
year = "2009",
month = "11",
doi = "10.1002/jbio.200910041",
language = "English (US)",
volume = "2",
pages = "656--668",
journal = "Journal of Biophotonics",
issn = "1864-063X",
publisher = "Wiley-VCH Verlag",
number = "11",

}

TY - JOUR

T1 - Label-free biochemical characterization of stem cells using vibrational spectroscopy

AU - Chan, James W

AU - Lieu, Deborah

PY - 2009/11

Y1 - 2009/11

N2 - Raman and infrared (IR) spectroscopy are two complementary vibrational spectroscopic techniques that have experienced a tremendous growth in their use in biological and biomedical research. This is, in large part, due to their unique capability of providing label-free intrinsic chemical information of living biological samples at tissue, cellular, or sub-cellular resolutions. This article reviews recent developments in applying these techniques for the characterization of stem cells. A discussion of the potential for these methods to address some of the major challenges in stem cell research is presented, as well as the technological and scientific advancements that are needed to progress the knowledge in the field.

AB - Raman and infrared (IR) spectroscopy are two complementary vibrational spectroscopic techniques that have experienced a tremendous growth in their use in biological and biomedical research. This is, in large part, due to their unique capability of providing label-free intrinsic chemical information of living biological samples at tissue, cellular, or sub-cellular resolutions. This article reviews recent developments in applying these techniques for the characterization of stem cells. A discussion of the potential for these methods to address some of the major challenges in stem cell research is presented, as well as the technological and scientific advancements that are needed to progress the knowledge in the field.

KW - Adult stem cells

KW - Embryonic stem cells

KW - Infrared spectroscopy

KW - Mesenchymal stem cells

KW - Raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=74049084523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74049084523&partnerID=8YFLogxK

U2 - 10.1002/jbio.200910041

DO - 10.1002/jbio.200910041

M3 - Article

C2 - 19653219

AN - SCOPUS:74049084523

VL - 2

SP - 656

EP - 668

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 11

ER -