Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging

Cai Li, Jeny Shklover, Mojtaba Parvizi, Benjamin E. Sherlock, Alba Alfonso Garcia, Anne K. Haudenschild, Leigh G. Griffiths, Laura Marcu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young’s Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine.

Original languageEnglish (US)
Pages (from-to)1870-1881
Number of pages12
JournalAnnals of Biomedical Engineering
Volume46
Issue number11
DOIs
StatePublished - Nov 15 2018

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Labels
Collagen
Fluorescence
Imaging techniques
Biomaterials
Degradation
Tissue regeneration
Monitoring
Tensile strength
Elastic moduli
Tissue
Proteins
Molecules
Regenerative Medicine

Keywords

  • Collagenase degradation
  • Fluorescence lifetime imaging
  • Non-destructive monitoring

ASJC Scopus subject areas

  • Biomedical Engineering

Cite this

Li, C., Shklover, J., Parvizi, M., Sherlock, B. E., Alfonso Garcia, A., Haudenschild, A. K., ... Marcu, L. (2018). Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging. Annals of Biomedical Engineering, 46(11), 1870-1881. https://doi.org/10.1007/s10439-018-2087-6

Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging. / Li, Cai; Shklover, Jeny; Parvizi, Mojtaba; Sherlock, Benjamin E.; Alfonso Garcia, Alba; Haudenschild, Anne K.; Griffiths, Leigh G.; Marcu, Laura.

In: Annals of Biomedical Engineering, Vol. 46, No. 11, 15.11.2018, p. 1870-1881.

Research output: Contribution to journalArticle

Li, C, Shklover, J, Parvizi, M, Sherlock, BE, Alfonso Garcia, A, Haudenschild, AK, Griffiths, LG & Marcu, L 2018, 'Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging', Annals of Biomedical Engineering, vol. 46, no. 11, pp. 1870-1881. https://doi.org/10.1007/s10439-018-2087-6
Li, Cai ; Shklover, Jeny ; Parvizi, Mojtaba ; Sherlock, Benjamin E. ; Alfonso Garcia, Alba ; Haudenschild, Anne K. ; Griffiths, Leigh G. ; Marcu, Laura. / Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging. In: Annals of Biomedical Engineering. 2018 ; Vol. 46, No. 11. pp. 1870-1881.
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