Label-free and direct protein detection on 3D plasmonic nanovoid structures using surface-enhanced Raman scattering

Mehmet Kahraman, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this paper we use surface-enhanced Raman spectroscopy (SERS) on 3D metallic structures for label-free detection and characterization of proteins of interest at low concentrations. The substrates are prepared via nanopatterning with latex nano/microparticles and Cr and Ag sputtering, yielding stable, tunable, and mechanically flexible plasmonic structures. The nanovoids generate a SERS signal of the proteins of interest that is background free and independent of the protein charge. Concentrations as low as 0.05μgmL-1 could be detected for 4 different proteins. The proteins also exhibit significantly different SERS spectra on these substrates, which is an important feature for future label-free direct detection schemes.

Original languageEnglish (US)
Pages (from-to)74-81
Number of pages8
JournalAnalytica Chimica Acta
Volume856
DOIs
StatePublished - Jan 26 2015

Fingerprint

Raman Spectrum Analysis
Raman scattering
Labels
scattering
Raman spectroscopy
protein
Proteins
substrate
Flexible structures
Latex
Substrates
Sputtering
detection

Keywords

  • Label-free
  • Nanobowls
  • Protein detection
  • Surface enhanced raman spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

@article{a63d6d5b95b14809bb163bcb2cc85092,
title = "Label-free and direct protein detection on 3D plasmonic nanovoid structures using surface-enhanced Raman scattering",
abstract = "In this paper we use surface-enhanced Raman spectroscopy (SERS) on 3D metallic structures for label-free detection and characterization of proteins of interest at low concentrations. The substrates are prepared via nanopatterning with latex nano/microparticles and Cr and Ag sputtering, yielding stable, tunable, and mechanically flexible plasmonic structures. The nanovoids generate a SERS signal of the proteins of interest that is background free and independent of the protein charge. Concentrations as low as 0.05μgmL-1 could be detected for 4 different proteins. The proteins also exhibit significantly different SERS spectra on these substrates, which is an important feature for future label-free direct detection schemes.",
keywords = "Label-free, Nanobowls, Protein detection, Surface enhanced raman spectroscopy",
author = "Mehmet Kahraman and Sebastian Wachsmann-Hogiu",
year = "2015",
month = "1",
day = "26",
doi = "10.1016/j.aca.2014.11.019",
language = "English (US)",
volume = "856",
pages = "74--81",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",

}

TY - JOUR

T1 - Label-free and direct protein detection on 3D plasmonic nanovoid structures using surface-enhanced Raman scattering

AU - Kahraman, Mehmet

AU - Wachsmann-Hogiu, Sebastian

PY - 2015/1/26

Y1 - 2015/1/26

N2 - In this paper we use surface-enhanced Raman spectroscopy (SERS) on 3D metallic structures for label-free detection and characterization of proteins of interest at low concentrations. The substrates are prepared via nanopatterning with latex nano/microparticles and Cr and Ag sputtering, yielding stable, tunable, and mechanically flexible plasmonic structures. The nanovoids generate a SERS signal of the proteins of interest that is background free and independent of the protein charge. Concentrations as low as 0.05μgmL-1 could be detected for 4 different proteins. The proteins also exhibit significantly different SERS spectra on these substrates, which is an important feature for future label-free direct detection schemes.

AB - In this paper we use surface-enhanced Raman spectroscopy (SERS) on 3D metallic structures for label-free detection and characterization of proteins of interest at low concentrations. The substrates are prepared via nanopatterning with latex nano/microparticles and Cr and Ag sputtering, yielding stable, tunable, and mechanically flexible plasmonic structures. The nanovoids generate a SERS signal of the proteins of interest that is background free and independent of the protein charge. Concentrations as low as 0.05μgmL-1 could be detected for 4 different proteins. The proteins also exhibit significantly different SERS spectra on these substrates, which is an important feature for future label-free direct detection schemes.

KW - Label-free

KW - Nanobowls

KW - Protein detection

KW - Surface enhanced raman spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84920406354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920406354&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2014.11.019

DO - 10.1016/j.aca.2014.11.019

M3 - Article

C2 - 25542360

AN - SCOPUS:84920406354

VL - 856

SP - 74

EP - 81

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

ER -