Label-free and direct protein detection on 3D plasmonic nanovoid structures using surface-enhanced Raman scattering

Mehmet Kahraman, Sebastian Wachsmann-Hogiu

Research output: Contribution to journalArticle

22 Scopus citations


In this paper we use surface-enhanced Raman spectroscopy (SERS) on 3D metallic structures for label-free detection and characterization of proteins of interest at low concentrations. The substrates are prepared via nanopatterning with latex nano/microparticles and Cr and Ag sputtering, yielding stable, tunable, and mechanically flexible plasmonic structures. The nanovoids generate a SERS signal of the proteins of interest that is background free and independent of the protein charge. Concentrations as low as 0.05μgmL-1 could be detected for 4 different proteins. The proteins also exhibit significantly different SERS spectra on these substrates, which is an important feature for future label-free direct detection schemes.

Original languageEnglish (US)
Pages (from-to)74-81
Number of pages8
JournalAnalytica Chimica Acta
StatePublished - Jan 26 2015



  • Label-free
  • Nanobowls
  • Protein detection
  • Surface enhanced raman spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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