L-type Ca2+ channel α(1c) subunit isoform switching in failing human ventricular myocardium

Yingbo Yang, Xiongwen Chen, Kenneth Margulies, Valvulan Jeevanandam, Pia Pollack, Beth A. Bailey, Steven R. Houser

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The objectives of this study were to determine the relative abundance of the L-type Ca channel α(1c) IVS3 isoforms that result from alternative splicing in normal human ventricular myocytes and to measure the changes in isoform expression in end stage heart failure. Methods: mRNA was isolated from left ventricular tissue and myocytes from non-failing and failing human hearts. RT-PCR with isoform-specific primers was used to obtain cDNAs that were then mutated for use in competitive PCR reactions. An RNase protection assay was also used to confirm the presence of one of the novel isoforms. Results: Four different α(1c) IVS3 isoforms were found in non-failing human ventricular myocytes using RT-PCR. Two isoforms contained exon 31 (termed IVS3A isoforms) and two isoforms contained exon 32 (termed IVS3B isoforms). One of these isoforms has not been observed previously and contains exon 31 and all but the last six base pairs of exon 32. In non-failing human ventricular myocytes the IVS3A isoform is 2.5 times more abundant than the IVS3B isoform. There were significant changes in the relative abundance of these isoforms in failing hearts, with the IVS3B isoform being twice as abundant as the IVS3A isoform. All isoforms were confirmed by RNase protection analysis. Conclusions: These experiments show that there are at least four L-type Ca channel mRNA isoforms in the normal human heart and that the relative abundance of these isoforms changes significantly in heart failure. These α(ic) isoform changes in heart failure are associated with dysfunctional electromechanical disturbances, but the specific physiological role of each L-type Ca channel isoform in normal and failing hearts needs to be defined. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)973-984
Number of pages12
JournalJournal of Molecular and Cellular Cardiology
Volume32
Issue number6
DOIs
StatePublished - 2000
Externally publishedYes

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Myocardium
Protein Isoforms
Muscle Cells
Exons
Heart Failure
Ribonucleases
Polymerase Chain Reaction
RNA Isoforms
Alternative Splicing
Base Pairing

Keywords

  • Ca channels
  • Congestive heart failure
  • Excitation-contraction coupling

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

L-type Ca2+ channel α(1c) subunit isoform switching in failing human ventricular myocardium. / Yang, Yingbo; Chen, Xiongwen; Margulies, Kenneth; Jeevanandam, Valvulan; Pollack, Pia; Bailey, Beth A.; Houser, Steven R.

In: Journal of Molecular and Cellular Cardiology, Vol. 32, No. 6, 2000, p. 973-984.

Research output: Contribution to journalArticle

Yang, Yingbo ; Chen, Xiongwen ; Margulies, Kenneth ; Jeevanandam, Valvulan ; Pollack, Pia ; Bailey, Beth A. ; Houser, Steven R. / L-type Ca2+ channel α(1c) subunit isoform switching in failing human ventricular myocardium. In: Journal of Molecular and Cellular Cardiology. 2000 ; Vol. 32, No. 6. pp. 973-984.
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abstract = "The objectives of this study were to determine the relative abundance of the L-type Ca channel α(1c) IVS3 isoforms that result from alternative splicing in normal human ventricular myocytes and to measure the changes in isoform expression in end stage heart failure. Methods: mRNA was isolated from left ventricular tissue and myocytes from non-failing and failing human hearts. RT-PCR with isoform-specific primers was used to obtain cDNAs that were then mutated for use in competitive PCR reactions. An RNase protection assay was also used to confirm the presence of one of the novel isoforms. Results: Four different α(1c) IVS3 isoforms were found in non-failing human ventricular myocytes using RT-PCR. Two isoforms contained exon 31 (termed IVS3A isoforms) and two isoforms contained exon 32 (termed IVS3B isoforms). One of these isoforms has not been observed previously and contains exon 31 and all but the last six base pairs of exon 32. In non-failing human ventricular myocytes the IVS3A isoform is 2.5 times more abundant than the IVS3B isoform. There were significant changes in the relative abundance of these isoforms in failing hearts, with the IVS3B isoform being twice as abundant as the IVS3A isoform. All isoforms were confirmed by RNase protection analysis. Conclusions: These experiments show that there are at least four L-type Ca channel mRNA isoforms in the normal human heart and that the relative abundance of these isoforms changes significantly in heart failure. These α(ic) isoform changes in heart failure are associated with dysfunctional electromechanical disturbances, but the specific physiological role of each L-type Ca channel isoform in normal and failing hearts needs to be defined. (C) 2000 Academic Press.",
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AB - The objectives of this study were to determine the relative abundance of the L-type Ca channel α(1c) IVS3 isoforms that result from alternative splicing in normal human ventricular myocytes and to measure the changes in isoform expression in end stage heart failure. Methods: mRNA was isolated from left ventricular tissue and myocytes from non-failing and failing human hearts. RT-PCR with isoform-specific primers was used to obtain cDNAs that were then mutated for use in competitive PCR reactions. An RNase protection assay was also used to confirm the presence of one of the novel isoforms. Results: Four different α(1c) IVS3 isoforms were found in non-failing human ventricular myocytes using RT-PCR. Two isoforms contained exon 31 (termed IVS3A isoforms) and two isoforms contained exon 32 (termed IVS3B isoforms). One of these isoforms has not been observed previously and contains exon 31 and all but the last six base pairs of exon 32. In non-failing human ventricular myocytes the IVS3A isoform is 2.5 times more abundant than the IVS3B isoform. There were significant changes in the relative abundance of these isoforms in failing hearts, with the IVS3B isoform being twice as abundant as the IVS3A isoform. All isoforms were confirmed by RNase protection analysis. Conclusions: These experiments show that there are at least four L-type Ca channel mRNA isoforms in the normal human heart and that the relative abundance of these isoforms changes significantly in heart failure. These α(ic) isoform changes in heart failure are associated with dysfunctional electromechanical disturbances, but the specific physiological role of each L-type Ca channel isoform in normal and failing hearts needs to be defined. (C) 2000 Academic Press.

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