L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow

P. K. Gopalan, C. W. Smith, H. Lu, E. Berg, L. V. Mclntirer, S. I. Simon

Research output: Contribution to journalArticle

Abstract

Neutrophil emigration across endothelial cells (EC) occurs in a sequential manner under shear conditions: rolling, firm adhesion, and transmigration. Rolling is mediated by selectins, while Firm adhesion and transmigration both require CD18 integrins. Activation is a prerequisite for CD 18 integrin function. However, the mechanisms by which CD18 integrins become activated upon contact with stimulated EC are not yet known. We have recently reported that cross-linking of L-selectin results in the rapid activation of CD18 integrin-dependent adhesion. We propose that under shear conditions, L-se!ectin ligation can signal CD 18 integrin activation that enables rolling neutrophils to adhere to endothelial surface receptors such asICAM-1 (CD54). Human CD54 and E-selectin (CD62E) were cotransfected into L cells where surface expression was comparable to that of cytokine-activated EC. Monolayers were placed in parallel plate flow chambers, and human neutrophil adhesion was evaluated at a wall shear stress of 2.0 dyn/cirf. Under these conditions, CD62E supported primary adhesion, and 23% of the roiling neutrophils stopped. The mean time from primary adhesion to stopping was 49 ±7 sec. Anti-CD54 monoclonal antibodies reduced stopping by 55%. When neutrophil L-selectin was cross-linked using a human chimera of DREG200 (that does not interact with FcRs on neutrophils) and goat anti-human F(ab% 49% of rolling neutrophils stopped within a mean of 15 ±5 sec. (pO.Ol). Anti-CD18 reduced this firm adhesion by 90%. These data support the conclusion that L-se!ectin can signal CD 18-dependent stationary adhesion of rolling neutrophils.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

Fingerprint

crosslinking
adhesion
neutrophils
Neutrophils
Adhesion
integrins
Integrins
Endothelial cells
shear stress
endothelial cells
L-Selectin
Endothelial Cells
Chemical activation
Selectins
E-Selectin
chimerism
Emigration and Immigration
Goats
Ligation
Shear stress

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Gopalan, P. K., Smith, C. W., Lu, H., Berg, E., Mclntirer, L. V., & Simon, S. I. (1996). L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow. FASEB Journal, 10(6).

L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow. / Gopalan, P. K.; Smith, C. W.; Lu, H.; Berg, E.; Mclntirer, L. V.; Simon, S. I.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Gopalan, PK, Smith, CW, Lu, H, Berg, E, Mclntirer, LV & Simon, SI 1996, 'L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow', FASEB Journal, vol. 10, no. 6.
Gopalan PK, Smith CW, Lu H, Berg E, Mclntirer LV, Simon SI. L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow. FASEB Journal. 1996;10(6).
Gopalan, P. K. ; Smith, C. W. ; Lu, H. ; Berg, E. ; Mclntirer, L. V. ; Simon, S. I. / L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
@article{49a7f5b8ad5e4dad9bc735fe81f44c36,
title = "L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow",
abstract = "Neutrophil emigration across endothelial cells (EC) occurs in a sequential manner under shear conditions: rolling, firm adhesion, and transmigration. Rolling is mediated by selectins, while Firm adhesion and transmigration both require CD18 integrins. Activation is a prerequisite for CD 18 integrin function. However, the mechanisms by which CD18 integrins become activated upon contact with stimulated EC are not yet known. We have recently reported that cross-linking of L-selectin results in the rapid activation of CD18 integrin-dependent adhesion. We propose that under shear conditions, L-se!ectin ligation can signal CD 18 integrin activation that enables rolling neutrophils to adhere to endothelial surface receptors such asICAM-1 (CD54). Human CD54 and E-selectin (CD62E) were cotransfected into L cells where surface expression was comparable to that of cytokine-activated EC. Monolayers were placed in parallel plate flow chambers, and human neutrophil adhesion was evaluated at a wall shear stress of 2.0 dyn/cirf. Under these conditions, CD62E supported primary adhesion, and 23{\%} of the roiling neutrophils stopped. The mean time from primary adhesion to stopping was 49 ±7 sec. Anti-CD54 monoclonal antibodies reduced stopping by 55{\%}. When neutrophil L-selectin was cross-linked using a human chimera of DREG200 (that does not interact with FcRs on neutrophils) and goat anti-human F(ab{\%} 49{\%} of rolling neutrophils stopped within a mean of 15 ±5 sec. (pO.Ol). Anti-CD18 reduced this firm adhesion by 90{\%}. These data support the conclusion that L-se!ectin can signal CD 18-dependent stationary adhesion of rolling neutrophils.",
author = "Gopalan, {P. K.} and Smith, {C. W.} and H. Lu and E. Berg and Mclntirer, {L. V.} and Simon, {S. I.}",
year = "1996",
language = "English (US)",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - L-SELECTIN cross-linking signals cd 18integrin-dependent firm adhesion under conditions of flow

AU - Gopalan, P. K.

AU - Smith, C. W.

AU - Lu, H.

AU - Berg, E.

AU - Mclntirer, L. V.

AU - Simon, S. I.

PY - 1996

Y1 - 1996

N2 - Neutrophil emigration across endothelial cells (EC) occurs in a sequential manner under shear conditions: rolling, firm adhesion, and transmigration. Rolling is mediated by selectins, while Firm adhesion and transmigration both require CD18 integrins. Activation is a prerequisite for CD 18 integrin function. However, the mechanisms by which CD18 integrins become activated upon contact with stimulated EC are not yet known. We have recently reported that cross-linking of L-selectin results in the rapid activation of CD18 integrin-dependent adhesion. We propose that under shear conditions, L-se!ectin ligation can signal CD 18 integrin activation that enables rolling neutrophils to adhere to endothelial surface receptors such asICAM-1 (CD54). Human CD54 and E-selectin (CD62E) were cotransfected into L cells where surface expression was comparable to that of cytokine-activated EC. Monolayers were placed in parallel plate flow chambers, and human neutrophil adhesion was evaluated at a wall shear stress of 2.0 dyn/cirf. Under these conditions, CD62E supported primary adhesion, and 23% of the roiling neutrophils stopped. The mean time from primary adhesion to stopping was 49 ±7 sec. Anti-CD54 monoclonal antibodies reduced stopping by 55%. When neutrophil L-selectin was cross-linked using a human chimera of DREG200 (that does not interact with FcRs on neutrophils) and goat anti-human F(ab% 49% of rolling neutrophils stopped within a mean of 15 ±5 sec. (pO.Ol). Anti-CD18 reduced this firm adhesion by 90%. These data support the conclusion that L-se!ectin can signal CD 18-dependent stationary adhesion of rolling neutrophils.

AB - Neutrophil emigration across endothelial cells (EC) occurs in a sequential manner under shear conditions: rolling, firm adhesion, and transmigration. Rolling is mediated by selectins, while Firm adhesion and transmigration both require CD18 integrins. Activation is a prerequisite for CD 18 integrin function. However, the mechanisms by which CD18 integrins become activated upon contact with stimulated EC are not yet known. We have recently reported that cross-linking of L-selectin results in the rapid activation of CD18 integrin-dependent adhesion. We propose that under shear conditions, L-se!ectin ligation can signal CD 18 integrin activation that enables rolling neutrophils to adhere to endothelial surface receptors such asICAM-1 (CD54). Human CD54 and E-selectin (CD62E) were cotransfected into L cells where surface expression was comparable to that of cytokine-activated EC. Monolayers were placed in parallel plate flow chambers, and human neutrophil adhesion was evaluated at a wall shear stress of 2.0 dyn/cirf. Under these conditions, CD62E supported primary adhesion, and 23% of the roiling neutrophils stopped. The mean time from primary adhesion to stopping was 49 ±7 sec. Anti-CD54 monoclonal antibodies reduced stopping by 55%. When neutrophil L-selectin was cross-linked using a human chimera of DREG200 (that does not interact with FcRs on neutrophils) and goat anti-human F(ab% 49% of rolling neutrophils stopped within a mean of 15 ±5 sec. (pO.Ol). Anti-CD18 reduced this firm adhesion by 90%. These data support the conclusion that L-se!ectin can signal CD 18-dependent stationary adhesion of rolling neutrophils.

UR - http://www.scopus.com/inward/record.url?scp=33748897530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748897530&partnerID=8YFLogxK

M3 - Article

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -