Kudoa thunni from blackfin tuna (Thunnus atlanticus) harvested off the island of St. Kitts, West Indies

Matt Griffin, Sylvie Quiniou, Cynthia Ware, Lewis Bogdanovic, Esteban Soto Martinez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Numerous myxozoan cysts (~1 mm) were found in the musculature of blackfin tuna (Thunnus atlanticus) harvested off the Caribbean island of St. Kitts. Myxospores were consistent with quadrate members of the Kudoidae, measuring 8.8 (8.2-9.4) μm wide, 7.3 (6.6-8.3) μm thick, and 6.2 (5.8-6.9) μm long with 4 uniform drop-like polar capsules measuring 2.7 (2.2-3.2) μm long and 2.0 (1.7-2.2) μm wide. The 18S small-subunit (SSU) and 28S large-subunit (LSU) ribosomal DNA sequences did not result in direct matches to any published sequences. However, the SSU sequences (1,786 base pairs [bp]) obtained from 6 individual cysts were identical and demonstrated high homology to Kudoa thunni (99.0%) from albacore (Thunnus alalunga). Alternatively, 33 unique sequences were obtained for the LSU (~800 bp), demonstrating 0.1 to 5.0% variability between them, although a majority of these sequences (60%) demonstrated high homology (>99%) to K. thunni. Morphologically, the case isolate was smaller than published descriptions of K. thunni; however, rDNA sequence homology, and phylogenetic placement based on concatenated SSU and LSU rDNA sequences suggests this case isolate and K. thunni are conspecific. To our knowledge this is the first report of K. thunni infection in blackfin tuna from the Caribbean.

Original languageEnglish (US)
Pages (from-to)110-116
Number of pages7
JournalJournal of Parasitology
Volume100
Issue number1
DOIs
StatePublished - Feb 2014
Externally publishedYes

Fingerprint

Kudoa
Thunnus alalunga
West Indies
Ribosomal DNA
homology
Islands
cyst
Base Pairing
Cysts
sequence homology
ribosomal DNA
Sequence Homology
nucleotide sequences
Capsules
phylogeny
infection
phylogenetics
DNA
Infection
Thunnus atlanticus

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)
  • Ecology, Evolution, Behavior and Systematics

Cite this

Kudoa thunni from blackfin tuna (Thunnus atlanticus) harvested off the island of St. Kitts, West Indies. / Griffin, Matt; Quiniou, Sylvie; Ware, Cynthia; Bogdanovic, Lewis; Soto Martinez, Esteban.

In: Journal of Parasitology, Vol. 100, No. 1, 02.2014, p. 110-116.

Research output: Contribution to journalArticle

Griffin, Matt ; Quiniou, Sylvie ; Ware, Cynthia ; Bogdanovic, Lewis ; Soto Martinez, Esteban. / Kudoa thunni from blackfin tuna (Thunnus atlanticus) harvested off the island of St. Kitts, West Indies. In: Journal of Parasitology. 2014 ; Vol. 100, No. 1. pp. 110-116.
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