Kinetics of transmission, infectivity, and genome stability of two novel mouse norovirus isolates in breeding mice

Jennifer A. Kelmenson, Darcy P. Pomerleau, Stephen M Griffey, Weidong Zhang, Michele J. Karolak, James R. Fahey

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Murine noroviruses are a recently discovered group of viruses found within mouse research colonies in many animal facilities worldwide. In this study, we used 2 novel mouse norovirus (MNV) wildtype isolates to examine the kinetics of transmission and tissue distribution in breeding units of NOD.CB17-Prkdc scid/J and backcrossed NOD.CB17-Prkdc scid/J × NOD/ShiLtJ (Nl) mice. Viral shedding in feces and dissemination to tissues of infected offspring mice were monitored by RT-PCR over a 6-wk period postpartum. Histologic sections of tissues from mice exposed to MNV were examined for lesions and their sera monitored for the presence of antibodies to MNV. Viruses shed in feces of parental and offspring mice were compared for sequence homology of the Orf 2 gene. Studies showed that the wildtype viruses MNV5 and MNV6 behaved differently in terms of the kinetics of transmission and distribution to tissues of offspring mice. For MNV5, virus transmission from parents to offspring was not seen before 3 wk after birth, and neither isolate was transmitted between cages of infected and control mice. Susceptibility to infection was statistically different between the 2 mouse strains used in the study. Both immunodeficient NOD.CB17-Prkdc scid/J mice and NOD. CB17-Prkdc scid/J × NOD/ShiLtJ offspring capable of mounting an immune response shed virus in their feces throughout the 6-wk study period, but no gross or histologic lesions were present in infected tissues. Progeny viruses isolated from the feces of infected offspring showed numerous mutations in the Orf2 gene for MNV5 but not MNV6. These results confirm previous studies demonstrating that the biology of MNV in mice varies substantially with each virus isolate and mouse strain infected.

Original languageEnglish (US)
Pages (from-to)27-36
Number of pages10
JournalComparative Medicine
Volume59
Issue number1
StatePublished - Feb 2009

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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    Kelmenson, J. A., Pomerleau, D. P., Griffey, S. M., Zhang, W., Karolak, M. J., & Fahey, J. R. (2009). Kinetics of transmission, infectivity, and genome stability of two novel mouse norovirus isolates in breeding mice. Comparative Medicine, 59(1), 27-36.