Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice

F. L. Oliveira, P. Frazão, R. Chammas, D. K. Hsu, Fu-Tong Liu, R. Borojevic, C. M. Takiya, M. C. El-Cheikh

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Galectin-3 (gal-3), a β-galactoside-binding animal lectin, plays a role in cell-cell and cell-extracellular matrix interactions. Extracellular gal-3 modulates cell migration and adhesion in several physiological and pathological processes. Gal-3 is highly expressed in activated macrophages. Schistosoma mansoni eggs display a large amount of gal-3 ligands on their surface and elicit a well-characterized, macrophage-dependent, granulomatous, inflammatory reaction. Here, we have investigated the acute and chronic phases of S. mansoni infection in wild-type and gal-3-/- mice. In the absence of gal-3, chronic-phase granulomas were smaller in diameter, displaying thinner collagen fibers with a loose orientation. Schistosoma-infected gal-3-/- mice had remarkable changes in the monocyte/macrophage, eosinophil, and B lymphocyte subpopulations as compared with the infected wild-type mice. We observed a reduction of macrophage number, an increase in eosinophil absolute number, and a decrease in B lymphocyte subpopulation (B220+/high cells) in the periphery during the evolution of the disease in gal-3-/- mice. B lymphopenia was followed by an increase of plasma cell number in bone marrow, spleen, and mesenteric lymph nodes of the infected gal-3-/- mice. The plasma IgG and IgE levels also increased in these mice. Gal-3 plays a role in the organization, collagen distribution, and mobilization of inflammatory cells to chronic-phase granulomas, niches for extramedullary myelopoiesis, besides interfering with monocyte-to-macrophage and B cell-to-plasma cell differentiation.

Original languageEnglish (US)
Pages (from-to)300-310
Number of pages11
JournalJournal of Leukocyte Biology
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2007

Fingerprint

Galectin 3
Schistosomiasis
Cell Differentiation
Macrophages
B-Lymphocytes
Lymphocyte Subsets
Plasma Cells
Granuloma
Eosinophils
Monocytes
Collagen
Myelopoiesis
Physiological Phenomena
Schistosoma
Galactosides
Schistosomiasis mansoni
Lymphopenia
Schistosoma mansoni
Pathologic Processes
Lectins

Keywords

  • Granuloma
  • Macrophages
  • Plasma cells
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice. / Oliveira, F. L.; Frazão, P.; Chammas, R.; Hsu, D. K.; Liu, Fu-Tong; Borojevic, R.; Takiya, C. M.; El-Cheikh, M. C.

In: Journal of Leukocyte Biology, Vol. 82, No. 2, 01.08.2007, p. 300-310.

Research output: Contribution to journalArticle

Oliveira, FL, Frazão, P, Chammas, R, Hsu, DK, Liu, F-T, Borojevic, R, Takiya, CM & El-Cheikh, MC 2007, 'Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice', Journal of Leukocyte Biology, vol. 82, no. 2, pp. 300-310. https://doi.org/10.1189/jlb.1206747
Oliveira, F. L. ; Frazão, P. ; Chammas, R. ; Hsu, D. K. ; Liu, Fu-Tong ; Borojevic, R. ; Takiya, C. M. ; El-Cheikh, M. C. / Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice. In: Journal of Leukocyte Biology. 2007 ; Vol. 82, No. 2. pp. 300-310.
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