Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice

F. L. Oliveira, P. Frazão, R. Chammas, D. K. Hsu, Fu-Tong Liu, R. Borojevic, C. M. Takiya, M. C. El-Cheikh

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Galectin-3 (gal-3), a β-galactoside-binding animal lectin, plays a role in cell-cell and cell-extracellular matrix interactions. Extracellular gal-3 modulates cell migration and adhesion in several physiological and pathological processes. Gal-3 is highly expressed in activated macrophages. Schistosoma mansoni eggs display a large amount of gal-3 ligands on their surface and elicit a well-characterized, macrophage-dependent, granulomatous, inflammatory reaction. Here, we have investigated the acute and chronic phases of S. mansoni infection in wild-type and gal-3-/- mice. In the absence of gal-3, chronic-phase granulomas were smaller in diameter, displaying thinner collagen fibers with a loose orientation. Schistosoma-infected gal-3-/- mice had remarkable changes in the monocyte/macrophage, eosinophil, and B lymphocyte subpopulations as compared with the infected wild-type mice. We observed a reduction of macrophage number, an increase in eosinophil absolute number, and a decrease in B lymphocyte subpopulation (B220+/high cells) in the periphery during the evolution of the disease in gal-3-/- mice. B lymphopenia was followed by an increase of plasma cell number in bone marrow, spleen, and mesenteric lymph nodes of the infected gal-3-/- mice. The plasma IgG and IgE levels also increased in these mice. Gal-3 plays a role in the organization, collagen distribution, and mobilization of inflammatory cells to chronic-phase granulomas, niches for extramedullary myelopoiesis, besides interfering with monocyte-to-macrophage and B cell-to-plasma cell differentiation.

Original languageEnglish (US)
Pages (from-to)300-310
Number of pages11
JournalJournal of Leukocyte Biology
Volume82
Issue number2
DOIs
StatePublished - Aug 1 2007

Keywords

  • Granuloma
  • Macrophages
  • Plasma cells
  • Schistosoma mansoni

ASJC Scopus subject areas

  • Cell Biology

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    Oliveira, F. L., Frazão, P., Chammas, R., Hsu, D. K., Liu, F-T., Borojevic, R., Takiya, C. M., & El-Cheikh, M. C. (2007). Kinetics of mobilization and differentiation of lymphohematopoietic cells during experimental murine schistosomiasis in galectin-3-/- mice. Journal of Leukocyte Biology, 82(2), 300-310. https://doi.org/10.1189/jlb.1206747