Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system

M. R. Sarantos, A. F H Lum, D. E. Staunton, S. I. Simon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Neutrophil capture on inflamed endothelium is controlled by dynamic regulation of the integrin CD11a/CD18 (LFA-1). Small molecules, antibodies, and certain divalent cations binding to specific epitopes on the integrin are able to stabilize either a closed (low affinity) or open (high affinity) state. To determine the relationship between LFA-1 conformation and affinity for ICAM-1 we assembled a cell-free system consisting of CD11a/CD18 heterodimer adhered to latex microspheres. The kinetics of dimeric ICAM-1 binding to the LFA-1 on the microspheres was measured via flow cytometry and a real time conformational shift into a lower affinity state was observed by addition of a small molecule inhibitor.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages4974-4977
Number of pages4
Volume26 VII
StatePublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

Other

OtherConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004
CountryUnited States
CitySan Francisco, CA
Period9/1/049/5/04

Fingerprint

Lymphocyte Function-Associated Antigen-1
Intercellular Adhesion Molecule-1
Microspheres
Epitopes
Integrins
Molecules
Kinetics
Flow cytometry
Latexes
Antibodies
Conformations
Positive ions
Divalent Cations
Latex

Keywords

  • Inflammation
  • Integrin conformation
  • Neutrophil adhesion

ASJC Scopus subject areas

  • Bioengineering

Cite this

Sarantos, M. R., Lum, A. F. H., Staunton, D. E., & Simon, S. I. (2004). Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 26 VII, pp. 4974-4977)

Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system. / Sarantos, M. R.; Lum, A. F H; Staunton, D. E.; Simon, S. I.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII 2004. p. 4974-4977.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sarantos, MR, Lum, AFH, Staunton, DE & Simon, SI 2004, Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 26 VII, pp. 4974-4977, Conference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004, San Francisco, CA, United States, 9/1/04.
Sarantos MR, Lum AFH, Staunton DE, Simon SI. Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII. 2004. p. 4974-4977
Sarantos, M. R. ; Lum, A. F H ; Staunton, D. E. ; Simon, S. I. / Kinetics of LFA-1 binding to ICAM-1 studied in a cell-free system. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 26 VII 2004. pp. 4974-4977
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