Kinetic studies of Ca2+ release from sarcoplasmic reticulum of normal and malignant hyperthermia susceptible pig muscles

Do Han Kim, Frank A. Sreter, S. Tsuyoshi Ohnishi, John F. Ryan, James Roberts, Paul D. Allen, Laszlo G. Meszaros, Bozena Antoniu, Noriaki Ikemoto

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

The time-course of Ca2+ release from sarcoplasmic reticulum isolated from muscles of normal pigs and those of pigs susceptible to malignant hyperthermia were investigated using stopped-flow spectrophotometry and arsenazo III as a Ca2+ indicator. Several methods were used to trigger Ca2+ release: (a) addition of halothane (e.g., 0.2 mM); (b) an increase of extravesicular Ca2+ concentration ([Ca0 2+]); (c) a combination of (a) and (b), and (d) replacement of ions (potassium gluconate with choline chloride) to produce membrane depolarization. The initial rates of Ca2+ release induced by either halothane or Ca2+ alone, or both, are at least 70% higher in malignant hyperthermic sarcoplasmic reticulum than in normal. The amount of Ca2+ released by halothane at low [Ca0 2+] in malignant hyperthermic sarcoplasmic reticulum is about twice as large as in normal sarcoplasmic reticulum. Membrane depolarization led to biphasic Ca2+ release in both malignant hyperthermic and normal sarcoplasmic reticulum, the rate constant of the rapid phase of Ca2+ release induced by membrane depolarization being significantly higher in malignant hyperthermic sarcoplasmic reticulum (k = 83 s-1) than in normal (k = 37 s-1). Thus, all types of Ca2+ release investigated (a, b, c and d) have higher rates in malignant hyperthermic sarcoplasmic reticulum than normal sarcoplasmic reticulum. These results suggest that the putative Ca2+ release channels located in the sarcoplasmic reticulum are altered in malignant hyperthermic sarcoplasmic reticulum.

Original languageEnglish (US)
Pages (from-to)320-327
Number of pages8
JournalBBA - Biomembranes
Volume775
Issue number3
DOIs
StatePublished - Sep 5 1984
Externally publishedYes

Keywords

  • (Porcine muscle)
  • Ca release
  • Halothane
  • Malignant hyperthermia
  • Membrane depolarization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Fingerprint Dive into the research topics of 'Kinetic studies of Ca<sup>2+</sup> release from sarcoplasmic reticulum of normal and malignant hyperthermia susceptible pig muscles'. Together they form a unique fingerprint.

  • Cite this

    Kim, D. H., Sreter, F. A., Ohnishi, S. T., Ryan, J. F., Roberts, J., Allen, P. D., Meszaros, L. G., Antoniu, B., & Ikemoto, N. (1984). Kinetic studies of Ca2+ release from sarcoplasmic reticulum of normal and malignant hyperthermia susceptible pig muscles. BBA - Biomembranes, 775(3), 320-327. https://doi.org/10.1016/0005-2736(84)90187-1