Abstract
The rice α-amylase isozymes Amy1A and Amy3D have distinct kinetic parameters and product profiles. For Amy3D, Michaelis constants (Km) decrease, and molecular activities (kcat) increase with the chain length of the substrates from maltose to maltoheptaose. Although Amy1A has a lower Km than Amy3D, the kcat of Amy1A was much lower than that of Amy3D. Amy1A produces only negligible amounts of glucose, while Amy3D produces glucose as a major product. Glucose regulates the expression of a number of genes in developing seedlings, so Amy3D may have a unique regulatory role in control of seedling development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-14 |
| Number of pages | 6 |
| Journal | Plant Science |
| Volume | 116 |
| Issue number | 1 |
| State | Published - 1996 |
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Keywords
- α-amylase
- Action pattern
- Carbon metabolite signal
- Kinetic constant
- Oligosaccharide
- Rice
ASJC Scopus subject areas
- Plant Science
- Biochemistry
- Biotechnology
Cite this
Kinetic parameters of two rice α-amylase isozymes for oligosaccharide degradation. / Terashima, Masaaki; Hayashi, Norio; Thomas, Bruce R.; Rodriguez, Raymond L.; Katoh, Shigeo.
In: Plant Science, Vol. 116, No. 1, 1996, p. 9-14.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinetic parameters of two rice α-amylase isozymes for oligosaccharide degradation
AU - Terashima, Masaaki
AU - Hayashi, Norio
AU - Thomas, Bruce R.
AU - Rodriguez, Raymond L.
AU - Katoh, Shigeo
PY - 1996
Y1 - 1996
N2 - The rice α-amylase isozymes Amy1A and Amy3D have distinct kinetic parameters and product profiles. For Amy3D, Michaelis constants (Km) decrease, and molecular activities (kcat) increase with the chain length of the substrates from maltose to maltoheptaose. Although Amy1A has a lower Km than Amy3D, the kcat of Amy1A was much lower than that of Amy3D. Amy1A produces only negligible amounts of glucose, while Amy3D produces glucose as a major product. Glucose regulates the expression of a number of genes in developing seedlings, so Amy3D may have a unique regulatory role in control of seedling development.
AB - The rice α-amylase isozymes Amy1A and Amy3D have distinct kinetic parameters and product profiles. For Amy3D, Michaelis constants (Km) decrease, and molecular activities (kcat) increase with the chain length of the substrates from maltose to maltoheptaose. Although Amy1A has a lower Km than Amy3D, the kcat of Amy1A was much lower than that of Amy3D. Amy1A produces only negligible amounts of glucose, while Amy3D produces glucose as a major product. Glucose regulates the expression of a number of genes in developing seedlings, so Amy3D may have a unique regulatory role in control of seedling development.
KW - α-amylase
KW - Action pattern
KW - Carbon metabolite signal
KW - Kinetic constant
KW - Oligosaccharide
KW - Rice
UR - http://www.scopus.com/inward/record.url?scp=0001129198&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001129198&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001129198
VL - 116
SP - 9
EP - 14
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 1
ER -