Kinetic characteristics of hamster (mesocricetus auratus) brown fat (Na+/K+)-ATPase

Effects of catecholamines

D. R. Hettinger, Barbara A Horwitz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

1. The (Na+/K+-ATPase activity of brown fat membranes is increased by norepinephrine, the physiological mediator of thermogenesis in this tissue. 2. This increased ATPase activity was inhibited approximately 50% by either propranolol (a β-adrenergic blocker) or phentolamine (an α-blocker). 3. The α-agonist, phenylephrine and the β-agonist, isoproterenol, also stimulated the ATPase activity. 4. That these latter effects were receptor-specific is supported by the finding that: (a) l(-)isoproterenol stimulation was inhibited by propranolol but not by phentolamine; (b) d(+)isoproterenol had no stimulatory effect on the ATPase activity; and (c) the l(-)phenylephrine-induced increase was inhibited by phentolamine but not by propranolol. 5. (-)norepinephrine, l(-)isoproterenol and l(-)phenylephrine all decreased the apparent Km for K+ of the (Na+/K+-ATPase but did not alter the apparent Kmfor ATP or the Vmax of the reaction.

Original languageEnglish (US)
Pages (from-to)355-360
Number of pages6
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume74
Issue number2
DOIs
StatePublished - 1983

Fingerprint

Brown Adipose Tissue
Mesocricetus
Isoproterenol
Cricetinae
Catecholamines
Phentolamine
Phenylephrine
Propranolol
Adenosine Triphosphatases
Norepinephrine
Adrenergic Antagonists
Thermogenesis
Adenosine Triphosphate
Membranes
sodium-translocating ATPase

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Kinetic characteristics of hamster (mesocricetus auratus) brown fat (Na+/K+)-ATPase : Effects of catecholamines. / Hettinger, D. R.; Horwitz, Barbara A.

In: Comparative Biochemistry and Physiology. Part C, Comparative, Vol. 74, No. 2, 1983, p. 355-360.

Research output: Contribution to journalArticle

@article{629ce852129d42f9abe3a7141a9e73fd,
title = "Kinetic characteristics of hamster (mesocricetus auratus) brown fat (Na+/K+)-ATPase: Effects of catecholamines",
abstract = "1. The (Na+/K+-ATPase activity of brown fat membranes is increased by norepinephrine, the physiological mediator of thermogenesis in this tissue. 2. This increased ATPase activity was inhibited approximately 50{\%} by either propranolol (a β-adrenergic blocker) or phentolamine (an α-blocker). 3. The α-agonist, phenylephrine and the β-agonist, isoproterenol, also stimulated the ATPase activity. 4. That these latter effects were receptor-specific is supported by the finding that: (a) l(-)isoproterenol stimulation was inhibited by propranolol but not by phentolamine; (b) d(+)isoproterenol had no stimulatory effect on the ATPase activity; and (c) the l(-)phenylephrine-induced increase was inhibited by phentolamine but not by propranolol. 5. (-)norepinephrine, l(-)isoproterenol and l(-)phenylephrine all decreased the apparent Km for K+ of the (Na+/K+-ATPase but did not alter the apparent Kmfor ATP or the Vmax of the reaction.",
author = "Hettinger, {D. R.} and Horwitz, {Barbara A}",
year = "1983",
doi = "10.1016/0742-8413(83)90114-7",
language = "English (US)",
volume = "74",
pages = "355--360",
journal = "Comparative biochemistry and physiology. C: Comparative pharmacology",
issn = "0742-8413",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Kinetic characteristics of hamster (mesocricetus auratus) brown fat (Na+/K+)-ATPase

T2 - Effects of catecholamines

AU - Hettinger, D. R.

AU - Horwitz, Barbara A

PY - 1983

Y1 - 1983

N2 - 1. The (Na+/K+-ATPase activity of brown fat membranes is increased by norepinephrine, the physiological mediator of thermogenesis in this tissue. 2. This increased ATPase activity was inhibited approximately 50% by either propranolol (a β-adrenergic blocker) or phentolamine (an α-blocker). 3. The α-agonist, phenylephrine and the β-agonist, isoproterenol, also stimulated the ATPase activity. 4. That these latter effects were receptor-specific is supported by the finding that: (a) l(-)isoproterenol stimulation was inhibited by propranolol but not by phentolamine; (b) d(+)isoproterenol had no stimulatory effect on the ATPase activity; and (c) the l(-)phenylephrine-induced increase was inhibited by phentolamine but not by propranolol. 5. (-)norepinephrine, l(-)isoproterenol and l(-)phenylephrine all decreased the apparent Km for K+ of the (Na+/K+-ATPase but did not alter the apparent Kmfor ATP or the Vmax of the reaction.

AB - 1. The (Na+/K+-ATPase activity of brown fat membranes is increased by norepinephrine, the physiological mediator of thermogenesis in this tissue. 2. This increased ATPase activity was inhibited approximately 50% by either propranolol (a β-adrenergic blocker) or phentolamine (an α-blocker). 3. The α-agonist, phenylephrine and the β-agonist, isoproterenol, also stimulated the ATPase activity. 4. That these latter effects were receptor-specific is supported by the finding that: (a) l(-)isoproterenol stimulation was inhibited by propranolol but not by phentolamine; (b) d(+)isoproterenol had no stimulatory effect on the ATPase activity; and (c) the l(-)phenylephrine-induced increase was inhibited by phentolamine but not by propranolol. 5. (-)norepinephrine, l(-)isoproterenol and l(-)phenylephrine all decreased the apparent Km for K+ of the (Na+/K+-ATPase but did not alter the apparent Kmfor ATP or the Vmax of the reaction.

UR - http://www.scopus.com/inward/record.url?scp=0020589870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020589870&partnerID=8YFLogxK

U2 - 10.1016/0742-8413(83)90114-7

DO - 10.1016/0742-8413(83)90114-7

M3 - Article

VL - 74

SP - 355

EP - 360

JO - Comparative biochemistry and physiology. C: Comparative pharmacology

JF - Comparative biochemistry and physiology. C: Comparative pharmacology

SN - 0742-8413

IS - 2

ER -