Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.

R. J. Leslie, R. B. Hird, L. Wilson, J. R. McIntosh, J. M. Scholey

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.

Original languageEnglish (US)
Pages (from-to)2771-2775
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number9
StatePublished - May 1987
Externally publishedYes

Fingerprint

Kinesin
Spindle Apparatus
Sea Urchins
Cytoskeleton
Microtubules
Buffers
Coloring Agents
Embryonic Structures
Adenosine Triphosphate
Antibodies

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles. / Leslie, R. J.; Hird, R. B.; Wilson, L.; McIntosh, J. R.; Scholey, J. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 9, 05.1987, p. 2771-2775.

Research output: Contribution to journalArticle

Leslie, R. J. ; Hird, R. B. ; Wilson, L. ; McIntosh, J. R. ; Scholey, J. M. / Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles. In: Proceedings of the National Academy of Sciences of the United States of America. 1987 ; Vol. 84, No. 9. pp. 2771-2775.
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