Abstract
Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2771-2775 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 84 |
| Issue number | 9 |
| State | Published - May 1987 |
| Externally published | Yes |
ASJC Scopus subject areas
- General
- Genetics
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Cite this
Leslie, R. J., Hird, R. B., Wilson, L., McIntosh, J. R., & Scholey, J. M. (1987). Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles. Proceedings of the National Academy of Sciences of the United States of America, 84(9), 2771-2775.