Abstract
Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2771-2775 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 84 |
| Issue number | 9 |
| State | Published - May 1987 |
| Externally published | Yes |
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ASJC Scopus subject areas
- General
- Genetics
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Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles. / Leslie, R. J.; Hird, R. B.; Wilson, L.; McIntosh, J. R.; Scholey, J. M.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 9, 05.1987, p. 2771-2775.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Kinesin is associated with a nonmicrotubule component of sea urchin mitotic spindles.
AU - Leslie, R. J.
AU - Hird, R. B.
AU - Wilson, L.
AU - McIntosh, J. R.
AU - Scholey, J. M.
PY - 1987/5
Y1 - 1987/5
N2 - Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.
AB - Sea urchin embryos in second division have been lysed into microtubule-stabilizing buffers to yield mitotic cytoskeletons (MCSs) that consist of two mitotic spindles surrounded by a cortical array of filaments. Microtubules have been completely extracted from MCSs by incubation at 0 degrees C with Ca2+-containing buffer. An antibody to the microtubule translocator kinesin stains the spindles in MCSs and in MCSs treated with 5 mM ATP and also stains spindle-remnants of the MCSs after the microtubules have been extracted. We conclude that kinesin binds to a nonmicrotubule component in the mitotic spindle. Based on these results, we present several models of kinesin function in the spindle.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023339737&partnerID=8YFLogxK
M3 - Article
C2 - 3106977
AN - SCOPUS:0023339737
VL - 84
SP - 2771
EP - 2775
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 9
ER -